Murthy V K, Shipp J C
Diabetes. 1977 Mar;26(3):222-9. doi: 10.2337/diab.26.3.222.
The accumulation of triglycerides in the myocardium of nonketotic and overtly ketotic diabetic rats was studied. There was no increase in heart triglycerides of nonketotic rats taken off insulin treatment, although the rats exhibited several indices of diabetes. In nonketotic diabetic rats untreated with insulin, myocardial triglycerides repeatedly increased and declined to control levels. In severely ketotic rats, heart triglycerides increased about threefold and did not decline with time. In order to understand the mechanism of increase in myocardial triglycerides in ketotic diabetes, the biosynthesis of triglycerides was studied with heart homogenates. The total esterification of sn-glycero-3-phosphate was unaltered, but the synthesis of diglycerides and triglycerides was increased in the myocardium of the ketotic rat. On treatment of the diabetic rats with insulin, the synthesis of di- and triglycerides in heart homogenates reverted to control values. Thus the results of the present study demonstrate that (1) a persistent increase in myocardial triglyceride content was observed only in the ketotic diabetic rat and (2) increased synthesis of triglycerides is a factor in its accumulation in the myocardium of the ketotic rat.
研究了非酮症和明显酮症糖尿病大鼠心肌中甘油三酯的蓄积情况。停用胰岛素治疗的非酮症大鼠，尽管表现出多种糖尿病指标，但其心脏甘油三酯并未增加。未用胰岛素治疗的非酮症糖尿病大鼠，心肌甘油三酯反复升高并降至对照水平。在严重酮症大鼠中，心脏甘油三酯增加约三倍且未随时间下降。为了解酮症糖尿病中心肌甘油三酯增加的机制，用心脏匀浆研究了甘油三酯的生物合成。sn-甘油-3-磷酸的总酯化未改变，但酮症大鼠心肌中甘油二酯和甘油三酯的合成增加。用胰岛素治疗糖尿病大鼠后，心脏匀浆中甘油二酯和甘油三酯的合成恢复到对照值。因此，本研究结果表明：（1）仅在酮症糖尿病大鼠中观察到心肌甘油三酯含量持续增加；（2）甘油三酯合成增加是其在酮症大鼠心肌中蓄积的一个因素。
