Li Qiuyan, Jiang Lu, Chen Ni, Wang Xingzhi, Yao Jiajun, Su Zhien, Zhao Suqing
Department of Pharmaceutical Engineering, School of Biomedical and Pharmaceutical Sciences, Guangdong University of Technology, Guangzhou 510006, China.
Department of Biomedical Engineering, School of Biomedical and Pharmaceutical Sciences, Guangdong University of Technology, Guangzhou 510006, China.
Foods. 2025 Mar 18;14(6):1026. doi: 10.3390/foods14061026.
Melanoma is one of the most lethal cancers originating from melanocytes. Its incidence and mortality have been rising rapidly for several decades and have posed a serious threat to human health. Current melanoma treatments are hindered by the scope of application, low efficiency, high cost, and toxic side effects. Due to their affordability and minimal side effects, natural bioactive compounds derived from plants are promising candidates for melanoma treatment. This study aims to delve into the isolation, purification, and characterization of potato proteins and to explore their potential in melanoma treatment. Two potato proteins, patatin PP-1 and aspartate protease inhibitor PP-2, were isolated and purified by a newly developed method in this work, and their physicochemical properties were systematically characterized. Both potato proteins showed great antiproliferative activities and migration inhibition effects on melanoma cells. Meanwhile, Western blotting results illustrated that they could induce endogenous cell apoptosis by regulating the Bax/Bcl-2 pathway. Notably, aspartate protease inhibitor PP-2 demonstrated the best performance in inhibiting the growth and migration of melanoma cells, which might be attributed to the combined effect of its significant antioxidative activity and the inhibition effect of certain necessary protease activities in melanoma. This study provides valuable insights for developing nutraceuticals and therapeutic strategies against melanoma, which can lead to breakthroughs in melanoma treatment.
黑色素瘤是起源于黑素细胞的最致命癌症之一。几十年来，其发病率和死亡率一直在迅速上升，对人类健康构成了严重威胁。目前的黑色素瘤治疗受到应用范围、效率低、成本高和毒副作用等因素的阻碍。由于植物来源的天然生物活性化合物价格低廉且副作用最小，它们是治疗黑色素瘤的有前途的候选物。本研究旨在深入探讨马铃薯蛋白的分离、纯化和特性，并探索它们在黑色素瘤治疗中的潜力。在这项工作中，通过一种新开发的方法分离并纯化了两种马铃薯蛋白，即马铃薯块茎蛋白PP-1和天冬氨酸蛋白酶抑制剂PP-2，并系统地表征了它们的物理化学性质。两种马铃薯蛋白对黑色素瘤细胞均表现出很强的抗增殖活性和迁移抑制作用。同时，蛋白质印迹结果表明，它们可以通过调节Bax/Bcl-2途径诱导内源性细胞凋亡。值得注意的是，天冬氨酸蛋白酶抑制剂PP-2在抑制黑色素瘤细胞的生长和迁移方面表现最佳，这可能归因于其显著的抗氧化活性和对黑色素瘤中某些必需蛋白酶活性的抑制作用的综合效应。本研究为开发抗黑色素瘤的营养保健品和治疗策略提供了有价值的见解，有望在黑色素瘤治疗方面取得突破。
