Key Laboratory of Plant Resource Conservation and Germplasm lnnovation in Mountainous Region (Ministry of Education), College of Life Sciences, Institute of Agro-Bioengineering, Guizhou University, Guiyang 550025, China.
Molecules. 2024 May 26;29(11):2513. doi: 10.3390/molecules29112513.
is a crucial edible fungus used in tea fermentation. In the industrial fermentation process, the fungus experiences a low to high osmotic pressure environment. To explore the law of material metabolism changes during osmotic pressure changes, NaCl was used here to construct different osmotic pressure environments. Liquid chromatography-mass spectrometry (LC-MS) combined with multivariate analysis was performed to analyze the distribution and composition of under different salt concentrations. At the same time, the in vitro antioxidant activity was evaluated. The LC-MS metabolomics analysis revealed significant differences between three mycelium samples grown on media with and without NaCl concentrations of 8% and 18%. The contents of gibberellin A3, A124, and prostaglandin A2 related to mycelial growth and those of arabitol and fructose-1,6-diphosphate related to osmotic pressure regulation were significantly reduced at high NaCl concentrations. The biosynthesis of energy-related pantothenol and pantothenic acid and antagonism-related fluvastatin, aflatoxin, and alternariol significantly increased at high NaCl concentrations. Several antioxidant capacities of mycelia were directly related to osmotic pressure and exhibited a significant downward trend with an increase in environmental osmotic pressure. The aforementioned results indicate that adapts to changes in salt concentration by adjusting their metabolite synthesis. At the same time, a unique set of strategies was developed to cope with high salt stress, including growth restriction, osmotic pressure balance, oxidative stress response, antioxidant defense, and survival competition.
是一种在茶叶发酵中使用的重要食用真菌。在工业发酵过程中，真菌经历从低渗透压到高渗透压的环境变化。为了探索渗透压变化过程中物质代谢变化的规律，本研究采用 NaCl 构建不同渗透压环境。采用液相色谱-质谱（LC-MS）结合多元分析方法，分析不同盐浓度下 的分布和组成。同时，评估了体外抗氧化活性。LC-MS 代谢组学分析表明，在含有 8%和 18%NaCl 浓度的培养基中生长的三种 菌丝体样品之间存在显著差异。与菌丝体生长相关的赤霉素 A3、A124 和前列腺素 A2 以及与渗透压调节相关的阿拉伯糖醇和果糖-1,6-二磷酸的含量在高 NaCl 浓度下显著降低。与能量相关的泛酸和泛酸的生物合成以及与拮抗作用相关的 fluvastatin、黄曲霉毒素和 alternariol 在高 NaCl 浓度下显著增加。几种 菌丝体的抗氧化能力直接与渗透压有关，随着环境渗透压的增加呈显著下降趋势。上述结果表明， 通过调整其代谢产物的合成来适应盐浓度的变化。同时，开发了一套独特的策略来应对高盐胁迫，包括生长受限、渗透压平衡、氧化应激反应、抗氧化防御和生存竞争。
