Kostareli Maria-Myrto, Westerink Timo, Couillaud Gabriel, Peippo Maaria, Govers Francine, Weijers Dolf, Evangelisti Edouard
Institut Sophia Agrobiotech, UMR INRAE 1355, CNRS 7254, Université Côte d'Azur, Sophia Antipolis, Provence-Alpes-Côte d'Azur, France.
Laboratory of Phytopathology, Wageningen University, Wageningen, Gelderland, the Netherlands.
mBio. 2025 May 14;16(5):e0396624. doi: 10.1128/mbio.03966-24. Epub 2025 Apr 16.
Polarity establishment is crucial for development, cellular organization, and signaling in living organisms. In animals and plants, this process involves DIX domain-containing proteins (DDPs) that assemble into oligomers via head-to-tail DIX polymerization, facilitating localized protein aggregation. This study uncovers the unexpected diversity of DDPs within the SAR supergroup, characterizing four DDPs with novel domain combinations conserved in Stramenopiles and Alveolates. These proteins are predominantly found in micro-swimmers and species with a motile stage in their life cycle. We hypothesize that DDPs from these eukaryotic lineages may be involved in cell polarity-related processes, including those associated with motility. Our work provides insights for further investigations of DDPs in protists and will enable the development of evolution-informed control strategies against pathogens and parasites within this clade.
极性建立对于生物体的发育、细胞组织和信号传导至关重要。在动物和植物中，这一过程涉及含DIX结构域的蛋白质（DDPs），它们通过头对头的DIX聚合组装成寡聚体，促进局部蛋白质聚集。本研究揭示了SAR超群中DDPs出人意料的多样性，鉴定出四种具有新结构域组合的DDPs，这些组合在不等鞭毛类和囊泡虫类中保守。这些蛋白质主要存在于微型游动体以及生命周期中有游动阶段的物种中。我们推测，来自这些真核生物谱系的DDPs可能参与细胞极性相关过程，包括与运动性相关的过程。我们的工作为进一步研究原生生物中的DDPs提供了见解，并将有助于制定针对该类群内病原体和寄生虫的基于进化信息的控制策略。
