University of Cambridge, Sainsbury Laboratory (SLCU), Cambridge, U.K.
Mol Plant Microbe Interact. 2023 Jun;36(6):315-322. doi: 10.1094/MPMI-12-22-0251-R. Epub 2023 Jul 27.
The oomycete infects a wide range of tropical crops worldwide. Like other filamentous plant pathogens, it secretes effectors to colonize plant tissues. Here, we characterize FIRE, an RXLR effector that contains a canonical mode I 14-3-3 phospho-sensor-binding motif that is conserved in effectors of several species. FIRE is phosphorylated in planta and interacts with multiple 14-3-3 proteins. Binding is sensitive to the R18 14-3-3 inhibitor. FIRE promotes plant susceptibility and co-localizes with its target around haustoria. This work uncovers a new type of oomycete effector target mechanism. It demonstrates that substrate mimicry for 14-3-3 proteins is a cross-kingdom effector strategy used by both prokaryotic and eukaryotic plant pathogens to suppress host immunity. [Formula: see text] Copyright © 2023 The Author(s). This is an open access article distributed under the CC BY 4.0 International license.
疫霉属真菌可感染全球范围内的多种热带作物。与其他丝状植物病原体一样，它会分泌效应子来定植植物组织。在这里，我们描述了 FIRE，这是一种 RXLR 效应子，它含有一个经典的模式 I 14-3-3 磷酸感应结合基序，该基序在多个物种的效应子中保守。FIRE 在植物体内被磷酸化，并与多种 14-3-3 蛋白相互作用。结合对 R18 14-3-3 抑制剂敏感。FIRE 促进植物易感性，并与目标物在吸器周围共定位。这项工作揭示了一种新型的卵菌效应子靶标机制。它表明，14-3-3 蛋白的底物模拟是原核和真核植物病原体用于抑制宿主免疫的一种跨领域效应子策略。[公式：见正文]版权所有©2023 作者。这是一篇在 CC BY 4.0 国际许可证下发布的开放获取文章。
