Shen Junqi, Cai Zhengyi, Zhang Cheng, Feng Xinyue, Zhang Chenzhi, Zhao Huan, Yin Chuanlin, Wang Bo, Yu Xiaoping, Zhang Biao
Key Laboratory of Microbiological Metrology, Measurement & Bio-Product Quality Security, State Administration for Market Regulation, College of Life Sciences, China Jiliang University, Hangzhou 310018, China.
College of Life Sciences, China Jiliang University, Hangzhou 310018, China.
Foods. 2025 Mar 25;14(7):1132. doi: 10.3390/foods14071132.
Ciprofloxacin has been extensively utilized in aquaculture due to its remarkable efficacy in preventing and treating bacterial infections in fish animals. However, the widespread application of ciprofloxacin has led to significant residue accumulation, necessitating the development of rapid, sensitive and specific detection methods. In this study, we developed a novel dual-mode quantitative immunochromatographic assay based on a portable reader and a photothermal instrument, enabling on-site ciprofloxacin detection. Under optimized conditions, the portable reader mode (Mode 1) achieved a detection range of 0.1-100.0 ng/L with a limit of detection (LOD) of 0.1 ng/mL. The photothermal instrument mode (Mode 2) achieved a detection range of 0.1-500.0 ng/mL with an LOD of 0.1 ng/mL. The sensitivity and accuracy of the method were validated using an Enzyme-Linked Immunosorbent Assay. This developed method successfully detected ciprofloxacin residues in samples of , , , and , with satisfactory recovery rates. The results demonstrated excellent specificity and applicability across various fish product matrices, offering a reliable and efficient solution for the on-site monitoring of ciprofloxacin residues in fish products.
环丙沙星因其在预防和治疗鱼类动物细菌感染方面的显著功效而在水产养殖中被广泛使用。然而，环丙沙星的广泛应用导致了大量残留积累，因此需要开发快速、灵敏且特异的检测方法。在本研究中，我们基于便携式读数器和光热仪器开发了一种新型双模式定量免疫色谱分析法，能够实现现场环丙沙星检测。在优化条件下，便携式读数器模式（模式1）的检测范围为0.1 - 100.0 ng/L，检测限（LOD）为0.1 ng/mL。光热仪器模式（模式2）的检测范围为0.1 - 500.0 ng/mL，LOD为0.1 ng/mL。使用酶联免疫吸附测定法验证了该方法的灵敏度和准确性。该开发方法成功检测了[此处原文缺失具体样本类型]、[此处原文缺失具体样本类型]、[此处原文缺失具体样本类型]和[此处原文缺失具体样本类型]样本中的环丙沙星残留，回收率令人满意。结果表明该方法在各种鱼类产品基质中具有出色的特异性和适用性，为鱼类产品中环丙沙星残留的现场监测提供了可靠且高效的解决方案。
