Pila-Lacuta Shirly, Pauccar David, Rojas-Vargas Jorge, Rodríguez-Cruz Ulises E, Sierra José Luis, Castelán-Sánchez Hugo G, Quispe-Ricalde María Antonieta
Departamento de Biología, Facultad de Ciencias Biológicas, Universidad Nacional de San Antonio Abad del Cusco, Cusco, Perú.
Department of Biology, University of Western Ontario, London, Ontario, Canada.
PLoS One. 2025 Apr 16;20(4):e0320639. doi: 10.1371/journal.pone.0320639. eCollection 2025.
Halomonas elongata strain ml10562, was isolated from hypersaline that was collected from Acos Peru. Average Nucleotide Identity (ANI) and dDDH (digital DNA-DNA Hybridization) values between strain ml10562 and type strains of Halomonas elongata species were 71.0-78.4% and 18.8-21.5%, respectively. The draft genome, spanning 4,075,440 base pairs, has a GC content of 64.2% and contains 3,912 genes. Functional characterization revealed the strain's ability to tolerate and resist increasing concentrations of sodium arsenate, with a minimum inhibitory concentration of 25 mM. Bioinformatic analysis revealed the presence of two operons, arsR-arsH-arsB and arsJ-gapdh-arsC, in the genome of strain ml10562, which could play a crucial role in arsenic resistance through transporter-mediated mechanisms. Overall, these results emphasize the potential adaptability of H. elongata ml10562 to arsenic-containing environments and extend our understanding of bacterial arsenic resistance mechanisms, allowing promising applications in bioremediation.
长盐单胞菌菌株ml10562是从秘鲁阿科斯采集的高盐环境中分离得到的。菌株ml10562与长盐单胞菌属模式菌株之间的平均核苷酸同一性（ANI）和数字DNA-DNA杂交（dDDH）值分别为71.0-78.4%和18.8-21.5%。该草图基因组跨度为4,075,440个碱基对，GC含量为64.2%，包含3,912个基因。功能表征显示该菌株能够耐受和抵抗不断增加浓度的砷酸钠，最低抑菌浓度为25 mM。生物信息学分析表明，菌株ml10562的基因组中存在两个操纵子，arsR-arsH-arsB和arsJ-gapdh-arsC，它们可能通过转运蛋白介导的机制在抗砷过程中发挥关键作用。总体而言，这些结果强调了长盐单胞菌ml10562对含砷环境的潜在适应性，并扩展了我们对细菌抗砷机制的理解，有望在生物修复中得到应用。
