Li Xiaoyan, Lu Hegang, Wang Qian, Yang Haixia, Yang Hongpeng, Wu Jiang, Huang Haidong
Tianjin Agricultural University, Tianjin 300384, PR China.
Tianjin Academy of Agricultural Sciences, Tianjin 300112, PR China.
Int J Syst Evol Microbiol. 2022 Dec;72(12). doi: 10.1099/ijsem.0.005652.
A Gram-stain-negative, aerobic and rod-shaped bacterium, strain Y2R2, was isolated from a saline-alkali soil sample collected from Binhai New Area, Tianjin, PR China. Growth of strain Y2R2 was observed at 10-45 °C (optimum, 30 °C), at pH 6.0-11.0 (optimum, pH 9.0) and in the presence of 0-15 % (w/v) NaCl (optimum, 9.0 %). Phylogenetic analysis based on 16S rRNA gene sequences showed that strain Y2R2 was affiliated with the genus and showed the highest similarity to BJGMM-B45 (99.0%) and DSM 4740 (98.4%). The digital DNA-DNA hybridization and average nucleotide identity values of 21.0-22.8 % and 73.3-75.7 % with the closely related species BJGMM-B45, DSM 4740, AL12, N12 and SYSU ZJ2214 were lower than the threshold recommended for species discrimination.The major respiratory quinone of strain Y2R2 was Q-9 and the major cellular fatty acids consisted of C, C cyclo ω8 and summed feature 8 (C ω7 and/or C ω6). The DNA G+C content of strain Y2R2 was 57.0 mol%. On the basis of this polyphasic taxonomic study, strain Y2R2 is considered to represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is Y2R2 (=CGMCC 1.16974=KCTC 72578).
从中国天津市滨海新区采集的盐碱土样本中分离出一株革兰氏染色阴性、需氧的杆状细菌，菌株Y2R2。在10-45°C（最适温度为30°C）、pH 6.0-11.0（最适pH为9.0）以及0-15%（w/v）NaCl（最适浓度为9.0%）条件下观察到菌株Y2R2的生长。基于16S rRNA基因序列的系统发育分析表明，菌株Y2R2隶属于该属，与BJGMM-B45（99.0%）和DSM 4740（98.4%）相似度最高。与密切相关物种BJGMM-B45、DSM 4740、AL12、N12和SYSU ZJ2214的数字DNA-DNA杂交和平均核苷酸同一性值分别为21.0-22.8%和73.3-75.7%，低于物种鉴定推荐的阈值。菌株Y2R2的主要呼吸醌为Q-9，主要细胞脂肪酸由C、C环ω8和总和特征8（C ω7和/或C ω6）组成。菌株Y2R2的DNA G+C含量为57.0 mol%。基于这项多相分类研究，菌株Y2R2被认为代表了该属的一个新物种，为此提出新物种名为sp. nov.。模式菌株为Y2R2（=CGMCC 1.16974=KCTC 72578）。
