Komara Ira, Andanawari Furi, Susanto Agus, Yuslianti Euis Reni, Hendiani Ina, Metta Prajna, Amaliya Amaliya
Department of Periodontics, Faculty of Dentistry, Universitas Padjadjaran, Bandung, Indonesia.
Post-Graduate Program in Periodontology, Dental Faculty, Universitas Padjadjaran, Bandung, Indonesia.
Eur J Dent. 2025 Jul;19(3):835-842. doi: 10.1055/s-0044-1801305. Epub 2025 Apr 16.
The aim of this study was to examine the biodegradation, angiogenesis, and inflammatory response in collagen-chitosan-polyvinyl alcohol (PVA) membranes.This study employed an experimental approach utilizing a randomized controlled trial design. Wistar rats were used as subjects, with 51 rats divided into three groups. Each group received a different treatment: application of the collagen-chitosan-PVA membrane, pericardial membrane, or cross-linked pericardial membrane, administered subcutaneously. On days 0, 7, 14, and 30, the rats were terminated, and the membranes and surrounding tissues were collected for analysis. A histological examination was performed to evaluate the membrane biodegradation rate, the number of blood vessels formed, and the inflammatory response.The data were analyzed using the Kruskal-Wallis and Mann-Whitney tests, with a -value of < 0.05 considered statistically significant.The collagen-chitosan-PVA membrane remained in the tissue up to day 30, while the pericardial membrane and cross-linked pericardial membrane were completely degraded by day 7. The average number of new blood vessels formed in the collagen-chitosan-PVA membrane on days 7, 14, and 30 was greater than that in the pericardial membrane and cross-linked pericardial membrane, which was statistically significant ( < 0.005). The average number of inflammatory cells in the collagen-chitosan-PVA membrane on day 30 was lower than that in the pericardial membrane and cross-linked pericardial membrane, which was statistically significant ( < 0.005) for neutrophils, monocytes, and lymphocytes. However, the difference was not statistically significant ( > 0.05) for eosinophils and mast cells.Biodegradation, angiogenesis, and the inflammatory response in collagen-chitosan-PVA membranes showed better results compared with other membranes. Collagen-chitosan-PVA membranes exhibit potential for application in guided tissue regeneration treatment for periodontal disease.
本研究的目的是检测胶原 - 壳聚糖 - 聚乙烯醇（PVA）膜的生物降解、血管生成及炎症反应。本研究采用随机对照试验设计的实验方法。以Wistar大鼠为研究对象，将51只大鼠分为三组。每组接受不同的处理：皮下注射胶原 - 壳聚糖 - PVA膜、心包膜或交联心包膜。在第0、7、14和30天，处死大鼠，收集膜及周围组织进行分析。进行组织学检查以评估膜的生物降解率、形成的血管数量及炎症反应。数据采用Kruskal - Wallis和Mann - Whitney检验进行分析，P值<0.05被认为具有统计学意义。胶原 - 壳聚糖 - PVA膜在组织中留存至第30天，而心包膜和交联心包膜在第7天完全降解。在第7、14和30天，胶原 - 壳聚糖 - PVA膜中形成的新生血管平均数量多于心包膜和交联心包膜，具有统计学意义（P<0.005）。在第30天，胶原 - 壳聚糖 - PVA膜中炎症细胞的平均数量低于心包膜和交联心包膜，对于中性粒细胞、单核细胞和淋巴细胞具有统计学意义（P<0.005）。然而，对于嗜酸性粒细胞和肥大细胞，差异无统计学意义（P>0.05）。与其他膜相比，胶原 - 壳聚糖 - PVA膜的生物降解、血管生成及炎症反应表现出更好的结果。胶原 - 壳聚糖 - PVA膜在牙周病引导组织再生治疗中具有应用潜力。
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