Zhou Tian, Chen Siwei, Ding Xinxin, Hu Zhihuan, Cen Lian, Zhang Xiaomeng
Department of Oral Maxillofacial-Head and Neck Oncology, Shanghai Ninth People's Hospital, College of Stomatology, Shanghai Jiao Tong University School of Medicine, Shanghai, China.
National Clinical Research Center for Oral Diseases, Shanghai Key Laboratory of Stomatology and Shanghai Research Institute of Stomatology, Shanghai, China.
Front Bioeng Biotechnol. 2021 Jun 9;9:630977. doi: 10.3389/fbioe.2021.630977. eCollection 2021.
Guided tissue regeneration (GTR) is a promising treatment for periodontal tissue defects, which generally uses a membrane to build a mechanical barrier from the gingival epithelium and hold space for the periodontal regeneration especially the tooth-supporting bone. However, existing membranes possess insufficient mechanical properties and limited bioactivity for periodontal bone regenerate. Herein, fish collagen and polyvinyl alcohol (Col/PVA) dual-layer membrane were developed via a combined freezing/thawing and layer coating method. This dual-layer membrane had a clear but contact boundary line between collagen and PVA layers, which were both hydrophilic. The dual membrane had an elongation at break of 193 ± 27% and would undergo an degradation duration of more than 17 days. Further cell experiments showed that compared with the PVA layer, the collagen layer not only presented good cytocompatibility with rat bone marrow-derived mesenchymal stem cells (BMSCs), but also promoted the osteogenic genes (RUNX2, ALP, OCN, and COL1) and protein (ALP) expression of BMSCs. Hence, the currently developed dual-layer membranes could be used as a stable barrier with a stable degradation rate and selectively favor the bone tissue to repopulate the periodontal defect. The membranes could meet the challenges encountered by GTR for superior defect repair, demonstrating great potential in clinical applications.
引导组织再生（GTR）是一种治疗牙周组织缺损的有前景的方法，它通常使用一种膜来构建与牙龈上皮的机械屏障，并为牙周再生尤其是牙齿支持骨保留空间。然而，现有的膜对于牙周骨再生而言机械性能不足且生物活性有限。在此，通过冷冻/解冻和层涂覆相结合的方法制备了鱼胶原蛋白和聚乙烯醇（Col/PVA）双层膜。这种双层膜在胶原蛋白层和聚乙烯醇层之间有一条清晰但相互接触的边界线，二者均为亲水性。该双层膜的断裂伸长率为193±27%，降解持续时间超过17天。进一步的细胞实验表明，与聚乙烯醇层相比，胶原蛋白层不仅与大鼠骨髓间充质干细胞（BMSCs）具有良好的细胞相容性，还能促进BMSCs的成骨基因（RUNX2、ALP、OCN和COL1）和蛋白质（ALP）表达。因此，目前开发的双层膜可作为一种具有稳定降解速率的稳定屏障，并选择性地有利于骨组织重新填充牙周缺损。这些膜能够应对GTR在卓越缺损修复方面遇到的挑战，在临床应用中显示出巨大潜力。
