Komara Ira, Susanto Agus, Amaliya Amaliya, Abbas Basril, Warastuti Yessy, Hendiani Ina, Miranda Aldilla, Erliani Annisa Pranuditha
Department of Periodontics, Faculty of Dentistry, Universitas Padjadjaran, Bandung, Indonesia.
Research Center for Radiation Process Technology, National Research and Innovation Agency (NRIA), Jakarta Indonesia.
Eur J Dent. 2023 May;17(2):530-538. doi: 10.1055/s-0042-1753451. Epub 2022 Oct 11.
The aim of this study was to evaluate the effect of gamma-ray irradiation on the physical, mechanical, and morphological characteristics of the polyvinyl alcohol (PVA)-collagen-chitosan membranes as a guided tissue regeneration membrane material.
The membrane was fabricated by mixing PVA, collagen, and chitosan using the film casting method. PVA-collagen-chitosan membranes were irradiated with various radiation dose (0, 15, and 25 kGy). Furthermore, it is characterized using Fourier-transform infrared (FTIR) for functional group identification, morphological test was performed using scanning electron microscopy (SEM), and mechanical properties (i.e., tensile strength and elongation) were evaluated using universal testing machine and swelling studies.
Statistical analysis was performed based on analysis of variance and post hoc with -value < 0.05.
The FTIR spectrum shows various peaks of functional groups from the PVA-collagen-chitosan membrane. The result of the statistical analysis show changes in tensile strength ( = 0.0004) and membrane elongation ( = 0.000451) at different radiation doses of 0, 15, and 25 kGy. The membrane absorption obtains -value of 0.0193, while the SEM results show that the PVA-collagen-chitosan membrane homogeneously mixed.
There is an effect of gamma-ray irradiation on tensile strength, elongation, and water absorption of the membranes. Increasing the radiation dose increases the value of tensile strength, while elongation and absorption of the membrane decrease. The PVA-collagen-chitosan membrane has the potential to develop as an alternative membrane for guided tissue regeneration.
本研究旨在评估γ射线辐照对作为引导组织再生膜材料的聚乙烯醇（PVA）-胶原蛋白-壳聚糖膜的物理、机械和形态学特性的影响。
采用流延法将PVA、胶原蛋白和壳聚糖混合制备膜。对PVA-胶原蛋白-壳聚糖膜进行不同辐射剂量（0、15和25 kGy）的辐照。此外，使用傅里叶变换红外光谱（FTIR）进行官能团鉴定，使用扫描电子显微镜（SEM）进行形态学测试，并使用万能试验机评估机械性能（即拉伸强度和伸长率）以及进行溶胀研究。
基于方差分析和事后检验进行统计分析，P值<0.05。
FTIR光谱显示了PVA-胶原蛋白-壳聚糖膜的各种官能团峰。统计分析结果表明，在0、15和25 kGy的不同辐射剂量下，拉伸强度（P = 0.0004）和膜伸长率（P = 0.000451）发生了变化。膜的吸水率P值为0.0193，而SEM结果表明PVA-胶原蛋白-壳聚糖膜均匀混合。
γ射线辐照对膜的拉伸强度、伸长率和吸水率有影响。增加辐射剂量会增加拉伸强度值，而膜的伸长率和吸水率会降低。PVA-胶原蛋白-壳聚糖膜有潜力开发成为引导组织再生的替代膜。
