Niwa M, Yuasa K, Kondo S, Sakuragawa N
Thromb Res. 1985 Jun 15;38(6):671-9. doi: 10.1016/0049-3848(85)90210-5.
Wakan-Yakus (traditional herbal drugs) such as Akyoh (Glutinum), Gaiyoh (Artemisiae folium), Sanshishi (Gardeniae fructus), Kizutsu (Aurantii fructus), and Taisoh (Zizyphi fructus) were studied in relation to their effects on blood coagulation-fibrinolysis. (1) All of the water extracts of the Wakan-Yakus prolonged aPTT and PT. The potency of the effectiveness on aPTT was in the order of Gaiyoh (Artemisiae folium) greater than Kizutsu (Aurantii fructus) greater than Sanshishi (Gardeniae fructus) greater than Taisoh (Zizyphi fructus) greater than Akyoh (Glutinum). (2) Gaiyoh (Artemisiae folium) greater than Kizutsu (Aurantii fructus) greater than Akyoh (Glutinum) greater than Taisoh (Zizyphi fructus) showed the antifibrinolytic effects in this order. On the other hand, Sanshishi showed the accelerating effect on fibrinolysis. (3) The inhibition modes of both thrombin and plasmin by Gaiyoh (Artemisiae folium) were shown to be competitive on Lineweaver-Burk plot. (4) Gaiyoh (Artemisiae folium) was gel-filtered on Sephadex G-25 column (1.5 X 90cm) equilibrated with distilled water at room temperature. Five fractions were obtained, and in the first to fourth fraction, strong anticoagulant effects on aPTT and PT were observed. We pooled first and second to make fraction I, and make fraction II from peak 3. The recovery rate was 4.2% by weight, and 36.7% by inhibition activity, and specific activity on the basis of inhibition to aPTT was 34.8% U/mg in the case with fraction II. Fraction I was found to be the same characteristically on blood coagulation. Fraction II was further purified by Sephadex LH-20 column (1.5 X 80 cm) at room temperature. Three fractions (Fraction IIa, IIb, IIc) were obtained, and the strong inhibitory effects was observed on both aPTT and PT in each fraction. The first fraction (fraction IIa) showed the strong inhibitory effect on aPTT, and the heightened specific activity with 17.6% as the recovery rate.
对和汉药（传统草药）如阿玉（糯米）、艾叶（艾叶）、山栀子（山栀子果实）、枳实（枳实果实）和大枣（大枣果实）对血液凝固 - 纤维蛋白溶解的影响进行了研究。（1）所有和汉药的水提取物均延长了活化部分凝血活酶时间（aPTT）和凝血酶原时间（PT）。对aPTT的有效作用强度顺序为：艾叶（艾叶）＞枳实（枳实果实）＞山栀子（山栀子果实）＞大枣（大枣果实）＞阿玉（糯米）。（2）艾叶（艾叶）＞枳实（枳实果实）＞阿玉（糯米）＞大枣（大枣果实）按此顺序显示出抗纤维蛋白溶解作用。另一方面，山栀子显示出对纤维蛋白溶解的促进作用。（3）在Lineweaver - Burk图上显示艾叶（艾叶）对凝血酶和纤溶酶的抑制模式均为竞争性。（4）艾叶（艾叶）在室温下用蒸馏水平衡的Sephadex G - 25柱（1.5×90cm）进行凝胶过滤。获得了五个馏分，在第一至第四馏分中，观察到对aPTT和PT有很强的抗凝作用。我们将第一和第二馏分合并制成馏分I，并从峰3制备馏分II。回收率按重量计为4.2%，按抑制活性计为36.7%，馏分II对aPTT抑制的比活性为34.8 U/mg。发现馏分I在血液凝固方面具有相同的特性。馏分II在室温下通过Sephadex LH - 20柱（1.5×80cm）进一步纯化。获得了三个馏分（馏分IIa、IIb、IIc），并且在每个馏分中均观察到对aPTT和PT有很强的抑制作用。第一个馏分（馏分IIa）对aPTT显示出很强的抑制作用，回收率为17.6%时比活性升高。
