Comparison of plateletapheresis with two continuous-flow cell separators using identical donors.

Each of 20 donors underwent plateletapheresis on both the CS-3000 (Fenwal) and the 2997 (IBM) blood cell separators. The same operator performed both procedures and conducted all product studies for each donor. Platelet collection efficiency was significantly greater with the 2997 (81%) than the CS-3000 (60%) (p less than .0005). Both techniques provided high platelet yields that were not significantly different (2997: 6.1 X 10(11), CS-3000: 5.7 X 10(11)), permitting comparison of other variables without accounting for different yields. Significantly more leukocytes were present in products collected with the CS-3000 (0.46 X 10(9)) than the 2997 (0.09 X 10(9), p less than 0.0005). More acid-citrate-dextrose was infused during procedures with the 2997 (471 ml) than the CS-3000 (324 ml, p less than 0.0005); the donors' ionized calcium decreased significantly more during procedures with the 2997 (27.3%) than the CS-3000 (17.0%, p less than 0.0005). Hypocalcemic symptoms were more common during procedures with the 2997. Product volume was greater with the 2997 (414 ml) than the CS-3000 (237 ml, p less than 0.0005). All products contained less than 1 ml of red cells. Set-up time was significantly greater with the 2997 (37 minutes) than the CS-3000 (18 minutes, p less than 0.0005), but processing time was significantly less with the 2997 (90 minutes) than the CS-3000 (110 minutes, p less than 0.0005). There was no difference in the decrease in donor platelet count with procedures between the 2997 (27%) and the CS-3000 (26%).(ABSTRACT TRUNCATED AT 250 WORDS)