Mintz P D, Cullis H M, Pearson T H
Transfusion. 1987 Mar-Apr;27(2):159-61. doi: 10.1046/j.1537-2995.1987.27287150189.x.
It is desirable to minimize the contamination of plateletapheresis products by lymphocytes because of the role these cells may play in febrile nonhemolytic transfusion reactions, alloimmunization, platelet deterioration during storage and also to reduce donor lymphocyte loss. This study attempted to determine whether lymphocyte contamination could be reduced by rapidly depleting plasma from the separation chamber at selected intervals during plateletapheresis with a blood cell separator (CS-3000, Fenwal). Donors who provided platelet components with more than 0.10 X 10(9) lymphocytes without rapid depletion underwent a second plateletapheresis procedure in which the rapid depletion technique was used. The plasma pump was reprogrammed to remove rapidly 12 ml of plasma from the separation chamber (pump speed 48 ml/min) immediately after the plasma pump reversal. All other collection techniques were identical for all procedures. Significantly fewer lymphocytes were collected in those procedures in which rapid depletion was used (mean, 0.187 X 10(9] than when it was not used (mean, 0.331 X 10(9] (p = 0.001, n = 30). There was no effect on platelet yield, efficiency of platelet collection, processing time, or total collection time. This procedure provides a product that should be considered for routine use.
由于淋巴细胞可能在发热性非溶血性输血反应、同种免疫、储存期间血小板变质中发挥作用，并且为了减少供体淋巴细胞损失，所以希望将血小板单采产品中的淋巴细胞污染降至最低。本研究试图确定在使用血细胞分离机（CS - 3000，芬沃尔公司）进行血小板单采期间，在选定的间隔时间从分离腔快速去除血浆是否可以减少淋巴细胞污染。对于那些提供的血小板成分中淋巴细胞超过0.10×10⁹且未进行快速去除的供体，进行第二次血小板单采程序，其中使用快速去除技术。在血浆泵反转后，立即重新编程血浆泵以从分离腔快速去除12毫升血浆（泵速48毫升/分钟）。所有其他采集技术在所有程序中均相同。在使用快速去除的程序中收集的淋巴细胞明显少于未使用快速去除的程序（平均值分别为0.187×10⁹和0.331×10⁹）（p = 0.001，n = 30）。对血小板产量、血小板采集效率、处理时间或总采集时间没有影响。该程序提供了一种应考虑常规使用的产品。
