Thingujam Doni, Majeed Aqsa, Sivarathri Bala Subramanyam, Narayana Nisarga Kodadinne, Bista Mohan K, Cowart Katie E, Knight Adelle J, Pajerowska-Mukhtar Karolina M, Bheemanahalli Raju, Mukhtar M Shahid
Department of Biological Sciences, Clemson University, 132 Long Hall, Clemson, SC 29634, USA.
Department of Biology, University of Alabama at Birmingham, 3100 East Science Hall, 902 14th Street South, Birmingham, AL 35294, USA.
Int J Mol Sci. 2025 Mar 21;26(7):2878. doi: 10.3390/ijms26072878.
Rhizosphere microbiome exerts a significant role in plant health, influencing nutrient availability, disease resistance, and overall plant growth. Establishing a robust and efficient nodulation process is essential for optimal nitrogen fixation in legumes like soybeans. Different soybean genotypes exhibit variations in their rhizosphere microbiome, potentially impacting nitrogen fixation through nodulation. However, a detailed understanding of how specific soybean genotypes influence rhizosphere microbial communities and nodulation patterns remains limited. Our study aims to investigate the relationship between rhizosphere microbial abundance and plant growth in four soybean genotypes. We evaluated plant growth parameters, including biomass, leaf area, and stomatal conductance, and identified significant genotypic differences in nodulation. Specifically, genotypes PI 458505 and PI 603490 exhibited high levels of nodulation, while PI 605839A and PI 548400 displayed low nodulation. 16S rRNA gene amplicon sequencing revealed diverse bacterial communities in the rhizosphere, with as the dominant phylum. High-nodulation genotypes harbored more diverse microbial communities enriched with and , while low-nodulation genotypes showed higher abundances of and . Alpha and beta diversity analyses confirmed distinct microbial community structures between high- and low-nodulation groups. Our findings suggest that the rhizosphere microbiome significantly influences soybean growth and nodulation, highlighting the potential for genotype-driven strategies to enhance plant-microbe interactions and improve soybean productivity.
根际微生物群在植物健康中发挥着重要作用，影响养分有效性、抗病性和植物整体生长。建立强大而高效的结瘤过程对于大豆等豆科植物的最佳固氮至关重要。不同的大豆基因型在其根际微生物群中表现出差异，可能通过结瘤影响固氮。然而，对于特定大豆基因型如何影响根际微生物群落和结瘤模式的详细了解仍然有限。我们的研究旨在调查四种大豆基因型中根际微生物丰度与植物生长之间的关系。我们评估了植物生长参数，包括生物量、叶面积和气孔导度，并确定了结瘤方面显著的基因型差异。具体而言，基因型PI 458505和PI 603490表现出高水平的结瘤，而PI 605839A和PI 548400表现出低结瘤。16S rRNA基因扩增子测序揭示了根际中多样的细菌群落，其中 为优势门。高结瘤基因型拥有更多样化的微生物群落，富含 和 ，而低结瘤基因型显示 和 的丰度更高。α和β多样性分析证实了高结瘤组和低结瘤组之间不同的微生物群落结构。我们的研究结果表明，根际微生物群显著影响大豆生长和结瘤，突出了基因型驱动策略在增强植物-微生物相互作用和提高大豆生产力方面的潜力。
