Lu Liangruinan, Fan Songle, Qin Bi, Wang Jingang, Wang Lifeng, Liu Shizhong
College of Horticulture and Landscape Architecture, Northeast Agricultural University, Harbin 150006, China.
Key Laboratory of Biology and Genetic Resources of Rubber Tree, Ministry of Agriculture and Rural Affairs, Rubber Research Institute, Chinese Academy of Tropical Agricultural Sciences, Haikou 571101, China.
Int J Mol Sci. 2025 Apr 5;26(7):3422. doi: 10.3390/ijms26073422.
Dandelions possess a wide range of medicinal properties and demonstrate remarkable adaptability and tolerance to salinity and alkalinity. genes in plants are implicated in growth, differentiation, metabolism, and responses to both biotic and abiotic stresses. The function of MYB genes in dandelions, particularly the R2R3-MYB gene family, requires further investigation. In this study, we identified a total of 130 members of the dandelion R2R3-MYB gene family at the genome-wide level, all of which were mapped to eight dandelion chromosomes. MEME analysis revealed that TmR2R3-MYB proteins contain three conserved motifs. Phylogenetic analysis categorized all TmR2R3-MYBs into 29 subfamilies. Transcriptomic studies in different tissues indicated that TmR2R3-MYBs exhibit distinct expression patterns in different tissues, indicating their diverse functions in dandelions. Notably, from the S22 subfamily displayed the highest expression level in roots. Additionally, six representative TmR2R3-MYBs were selected from the S22 subfamily for expression profiling under salinity and alkalinity treatments. The results demonstrated that the TmR2R3-MYBs from the S22 subfamily are involved in the response to salinity and alkalinity stress. These findings provide a basis for further exploration of the functions of TmR2R3-MYBs in abiotic stress tolerance.
蒲公英具有广泛的药用特性，并且对盐碱化表现出显著的适应性和耐受性。植物中的基因与生长、分化、代谢以及对生物和非生物胁迫的反应有关。蒲公英中MYB基因的功能，特别是R2R3-MYB基因家族的功能，需要进一步研究。在本研究中，我们在全基因组水平上共鉴定出130个蒲公英R2R3-MYB基因家族成员，所有这些成员都定位到8条蒲公英染色体上。MEME分析表明，TmR2R3-MYB蛋白含有三个保守基序。系统发育分析将所有TmR2R3-MYB分为29个亚家族。不同组织的转录组学研究表明，TmR2R3-MYB在不同组织中表现出不同的表达模式，表明它们在蒲公英中具有多种功能。值得注意的是，来自S22亚家族的成员在根中表达水平最高。此外，从S22亚家族中选择了6个代表性的TmR2R3-MYB进行盐碱处理下的表达谱分析。结果表明，S22亚家族的TmR2R3-MYB参与了对盐碱胁迫的响应。这些发现为进一步探索TmR2R3-MYB在非生物胁迫耐受性中的功能提供了基础。
