Lee Min Yeong, Campomayor Nicole Bon, Kim Hee Jin, Kim Mikyung
Department of Convergence science, Sahmyook University, Hwarangro 815, Nowongu, Seoul, 01795, Republic of Korea.
Uimyung Research Institute for Neuroscience, Sahmyook University, Hwarangro 815, Nowongu, Seoul, 01795, Republic of Korea.
Genes Genomics. 2025 Apr 17. doi: 10.1007/s13258-025-01642-x.
As human life expectancy increases, maintaining a healthy lifestyle has become crucial. However, excessive exercise (EE) can lead to negative consequences such as muscle damage and exercise addiction. Recently, numerous reports have indicated that EE negatively impacts cognitive performance, although the exact mechanism remains unclear.
This study aimed to investigate the specific mechanisms underlying cognitive alterations induced by EE.
We conducted the Y-maze, Barnes maze, and Novel Object Recognition Test to assess both short-term and long-term memory, as well as object recognition ability. We then validated our findings using qRT-PCR to elucidate the underlying mechanisms. Additionally, Diclofenac (Dic), an anti-inflammatory drug, was administered to evaluate its effects on cognitive function and the results of the molecular experiments.
EE-induced mice exhibited cognitive impairments, along with elevated expression of inflammatory cytokines such as tumor necrosis factor-α, interleukin (IL) -6, and IL-1β, and downregulated monocarboxylate transporters (MCTs) like MCT4. However, animals pre-treated with Dic regained cognitive function, alongside restored levels of IL-6, IL-1β, and MCT4.
MCT4 plays may play a crucial role in EE-induced cognitive impairments.
随着人类预期寿命的增加，保持健康的生活方式变得至关重要。然而，过度运动（EE）会导致诸如肌肉损伤和运动成瘾等负面后果。最近，大量报告表明，过度运动对认知表现有负面影响，尽管确切机制尚不清楚。
本研究旨在探讨过度运动引起认知改变的具体机制。
我们进行了Y迷宫、巴恩斯迷宫和新物体识别测试，以评估短期和长期记忆以及物体识别能力。然后，我们使用qRT-PCR验证我们的发现，以阐明潜在机制。此外，给予双氯芬酸（Dic），一种抗炎药物，以评估其对认知功能和分子实验结果的影响。
过度运动诱导的小鼠表现出认知障碍，同时肿瘤坏死因子-α、白细胞介素（IL）-6和IL-1β等炎性细胞因子的表达升高，而单羧酸转运体（MCTs）如MCT4的表达下调。然而，预先用双氯芬酸治疗的动物恢复了认知功能，同时IL-6、IL-1β和MCT4的水平也恢复了。
MCT4可能在过度运动诱导的认知障碍中起关键作用。
