Sun Jing, Tang Shu Mei, Sun Jing, Gao Wei
Department Of Respiratory Medicine, University of Health and Rehabilitation Sciences (Qingdao Central Hospital), Qingdao, 266042, China.
Department Of PICC, University of Health and Rehabilitation Sciences (Qingdao Central Hospital), Qingdao, 266042, China.
Hereditas. 2025 Apr 17;162(1):65. doi: 10.1186/s41065-025-00426-3.
Lung cancer remains one of the leading causes of cancer-related death worldwide, with a significant number of patients succumbing to the disease each year. Olea europaea, commonly known as the olive tree, offers a range of health benefits due to its rich content of antioxidants. In the present study, we have reported the green synthesis of a bimetallic nanocomposite of zinc and copper using the leaf extract of Olea europaea (Zn/Cu NCs@ Olea europaea). The nanoparticles were characterized using common chemical techniques. The antioxidant activity of Zn/Cu NCs@ Olea europaea was evaluated using the DPPH assay. The cytotoxicity and anti-lung cancer activity of Zn/Cu NCs@ Olea europaea were investigated using the MTT assay. The results of XRD analysis and FE-SEM imaging showed a crystalline structure for Zn/Cu NCs@ Olea europaea with a semi-spherical morphology and an average size of 49.37 nm. Zn/Cu NCs@ Olea europaea scavenged the free radical DPPH with an IC of 363.42 ± 5.02 µg/mL. Furthermore, Zn/Cu NCs@ Olea europaea exhibited acceptable anti-lung cancer activity by preventing growth in the cell lines SK-MES-1, A-549, and LK-2 with IC of 154.00 ± 1.83, 228.83 ± 10.59, and 250.55 ± 8.04 µg/mL respectively. The NPs were inactive against the normal cell lines of HUVEC even at high concentrations. The results of the study indicate that Zn/Cu NCs@ Olea europaea, which is green synthesized with a sufficient nano size, can be considered a potent anti-lung cancer agent.
肺癌仍然是全球癌症相关死亡的主要原因之一，每年有大量患者死于该疾病。油橄榄，通常被称为橄榄树，因其富含抗氧化剂而具有一系列健康益处。在本研究中，我们报道了使用油橄榄叶提取物绿色合成锌和铜的双金属纳米复合材料（Zn/Cu NCs@油橄榄）。使用常见化学技术对纳米颗粒进行了表征。使用DPPH测定法评估了Zn/Cu NCs@油橄榄的抗氧化活性。使用MTT测定法研究了Zn/Cu NCs@油橄榄的细胞毒性和抗肺癌活性。XRD分析和FE-SEM成像结果表明，Zn/Cu NCs@油橄榄具有晶体结构，呈半球形形态，平均尺寸为49.37nm。Zn/Cu NCs@油橄榄清除自由基DPPH的IC50为363.42±5.02μg/mL。此外，Zn/Cu NCs@油橄榄通过抑制SK-MES-1、A-549和LK-2细胞系的生长表现出可接受的抗肺癌活性，其IC50分别为154.00±1.83、228.83±10.59和250.55±8.04μg/mL。即使在高浓度下，这些纳米颗粒对HUVEC正常细胞系也无活性。研究结果表明，通过绿色合成且具有足够纳米尺寸的Zn/Cu NCs@油橄榄可被视为一种有效的抗肺癌药物。
