Sellami Hanen, Khan Shakeel Ahmad, Ahmad Ishaq, Alarfaj Abdullah A, Hirad Abdurahman H, Al-Sabri Ahmed E
Laboratory of Treatment and Valorization of Water Rejects, Water Research and Technologies Center (CERTE), Borj-Cedria Technopark, University of Carthage, Soliman 8020, Tunisia.
Center of Super-Diamond and Advanced Films (COSDAF), Department of Chemistry, City University of Hong Kong, 83 Tat Chee Avenue, Kowloon, Hong Kong 999077, China.
Int J Mol Sci. 2021 Nov 22;22(22):12562. doi: 10.3390/ijms222212562.
Herein, we report the green synthesis of silver nanoparticles (OE-Ag NPs) by ecofriendly green processes using biological molecules of leaf extract. Green synthesized OE-Ag NPs were successfully characterized using different spectroscopic techniques. Antibacterial activity of OE-Ag NPs was assessed against four different bacteriological strains using the dilution serial method. The cytotoxic potential was determined against MCF-7 carcinoma cells using MTT assay in terms of cell viability percentage. Antioxidant properties were evaluated in terms of 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging. Biocompatibility was further examined by incubating the synthesized NPs with hMSC cells for 24 h. The results were demonstrated that synthesized OE-Ag NPs presented excellent log reduction in the growth of all the tested bacterial strains, which as statistically equivalent ( > 0.05) to the standard antibiotic drug. Moreover, they also demonstrated excellent cytotoxic efficacy against the MCF-7 carcinoma cells compared to plant lead extract and Com-Ag NPs. Green synthesized OE-Ag NPs appeared more biocompatible to hMSC and 293T cells compared to Com-Ag NPs. Excellent biological results of the OE-Ag NPs might be attributed to the synergetic effect of NPs' properties and the adsorbed secondary metabolites of plant leaf extract. Hence, this study suggests that synthesized OE-Ag NPs can be a potential contender for their various biological and nutraceutical applications. Moreover, this study will open a new avenue to produce biocompatible nanoparticles with additional biological functionalities from the plants.
在此，我们报告了通过使用叶提取物的生物分子的生态友好型绿色工艺绿色合成银纳米颗粒（OE-Ag NPs）。使用不同的光谱技术成功表征了绿色合成的OE-Ag NPs。采用连续稀释法评估了OE-Ag NPs对四种不同细菌菌株的抗菌活性。使用MTT法根据细胞活力百分比测定了对MCF-7癌细胞的细胞毒性潜力。通过2,2-二苯基-1-苦基肼（DPPH）自由基清除来评估抗氧化性能。通过将合成的纳米颗粒与hMSC细胞孵育24小时进一步检测生物相容性。结果表明，合成的OE-Ag NPs对所有测试细菌菌株的生长均呈现出优异的对数减少，这在统计学上与标准抗生素药物等效（>0.05）。此外，与植物铅提取物和Com-Ag NPs相比，它们对MCF-7癌细胞也表现出优异的细胞毒性功效。与Com-Ag NPs相比，绿色合成的OE-Ag NPs对hMSC和293T细胞似乎更具生物相容性。OE-Ag NPs优异的生物学结果可能归因于纳米颗粒特性与植物叶提取物吸附的次生代谢产物的协同作用。因此，本研究表明，合成的OE-Ag NPs在其各种生物和营养应用中可能是一个潜在的竞争者。此外，本研究将开辟一条新途径，从植物中生产具有额外生物功能的生物相容性纳米颗粒。
