Maqbool Qaisar, Iftikhar Sidra, Nazar Mudassar, Abbas Fazal, Saleem Asif, Hussain Talib, Kausar Rizwan, Anwaar Sadaf, Jabeen Nyla
Interdisciplinary Research Organization, University of Chakwal (UOC), Chakwal, Pakistan.
Instituite of Molecular Biology and Biotechnology, The University of Lahore, Lahore, Pakistan.
IET Nanobiotechnol. 2017 Jun;11(4):463-468. doi: 10.1049/iet-nbt.2016.0125.
In present investigation, copper oxide (CuO) nanostructures have been prepared via green chemistry. leaf extract act as strong chelating agent for tailoring physical as well as bio-medical characteristics of CuO at the nano-size. Physical characterisation such as scanning electron microscope analysis depicts the formation of homogenised spherical shape nanoparticles (NPs) with average size of 42 nm. X-ray diffraction and Fourier transform infrared spectroscopy further confirmed the crystalline pure phase and monoclinic structure. High performance liquid chromatography (HPLC) testing is performed to evaluate the relative concentration of bioactive molecules in the leaf extract. From HPLC results capping action of organic molecules around CuO-NPs is hypothesised. The antimicrobial potency of biosynthesised CuO-NPs have been evaluated using colony forming unit (CFU) counting assay and disc diffusion method which shows a significant zone of inhibition against bacterial and fungal strains may be highly potential for future antimicrobial pharmaceutics. Furthermore, reduction of various precursors by plant extract will reduce environmental impact over chemical synthesis.
在目前的研究中，通过绿色化学制备了氧化铜（CuO）纳米结构。叶提取物作为一种强大的螯合剂，用于定制纳米尺寸CuO的物理和生物医学特性。扫描电子显微镜分析等物理表征描绘了平均尺寸为42 nm的均匀球形纳米颗粒（NPs）的形成。X射线衍射和傅里叶变换红外光谱进一步证实了结晶纯相和单斜结构。进行高效液相色谱（HPLC）测试以评估叶提取物中生物活性分子的相对浓度。根据HPLC结果推测了有机分子在CuO-NPs周围的封端作用。使用菌落形成单位（CFU）计数法和纸片扩散法评估了生物合成的CuO-NPs的抗菌效力，结果表明对细菌和真菌菌株有显著的抑制圈，这对于未来的抗菌药物可能具有很高的潜力。此外，植物提取物还原各种前体将减少对化学合成的环境影响。
