• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

卵巢特异性Mro-IR在淡水大虾卵原细胞分化和卵母细胞发育中的作用

Involvement of the ovarian-specific Mro-IR in oogonia differentiation and oocyte development in freshwater giant prawn .

作者信息

Ma Wen-Ming, Xu Hai-Jing

机构信息

College of Advanced Agricultural Sciences, Zhejiang Wanli University, Ningbo, Zhejiang, China.

出版信息

Front Endocrinol (Lausanne). 2025 Apr 3;16:1516849. doi: 10.3389/fendo.2025.1516849. eCollection 2025.

DOI:10.3389/fendo.2025.1516849
PMID:40248152
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC12004233/
Abstract

INTRODUCTION

Mro-IR is an insulin-like receptor and is uniquely expressed in the ovary of the freshwater giant prawn . However, the understanding of this conserved receptor involved in the molecular mechanism underpinning ovarian development and female reproduction of is still fragmentary.

METHODS

In the present study, knockdown of Mro-IR in the proliferative stage and premature stage of ovarian development in female prawn induced abnormal oogonia differentiation and disordered oocyte development.

RESULTS

The histological analysis showed that Mro-IR-silencing caused abnormal cellular morphology of some early vitellogenic oocytes (Oc2) and significantly delayed the proliferation of late vitellogenic oocytes (Oc3) in the proliferative stage of the ovary. Meanwhile, the Mro-IR silence led to the abnormal Oc3 with indistinct boundary and destructive structure of yolk accumulation in Oc3 in the premature stage of the ovary. Furthermore, to expound the potential roles of Mro-IR in ovarian development, a large amount of new data on significantly differentially upregulated and downregulated transcriptions was enriched, and the response of the primary Kyoto Encyclopedia of Genes and Genomes (KEGG) biological pathways was investigated. Their possible molecular regulatory relationships in gonad development and reproduction were briefly illustrated in the putative intuitive cascade regulation axis or networks.

DISCUSSION

This finding offered new insight regarding the mechanism of the IR gene family in ovarian development and reproduction of crustaceans.

摘要

引言

Mro-IR是一种类胰岛素受体，在淡水大对虾卵巢中特异性表达。然而，对于这种参与卵巢发育和雌性生殖分子机制的保守受体的了解仍不完整。

方法

在本研究中，对处于卵巢发育增殖期和早熟期的雌性对虾进行Mro-IR基因敲低，导致卵原细胞分化异常和卵母细胞发育紊乱。

结果

组织学分析表明，在卵巢增殖期，Mro-IR基因沉默导致一些早期卵黄发生期卵母细胞（Oc2）细胞形态异常，并显著延迟晚期卵黄发生期卵母细胞（Oc3）的增殖。同时，在卵巢早熟期，Mro-IR基因沉默导致Oc3边界不清且卵黄积累结构破坏的异常Oc3。此外，为阐明Mro-IR在卵巢发育中的潜在作用，富集了大量显著差异上调和下调转录的新数据，并研究了京都基因与基因组百科全书（KEGG）主要生物途径的响应。在假定的直观级联调控轴或网络中简要说明了它们在性腺发育和生殖中可能的分子调控关系。

讨论

这一发现为IR基因家族在甲壳类动物卵巢发育和生殖中的机制提供了新的见解。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/eb4f/12004233/c30a2dfce1f9/fendo-16-1516849-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/eb4f/12004233/c27f58051960/fendo-16-1516849-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/eb4f/12004233/8c6b0c92305f/fendo-16-1516849-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/eb4f/12004233/acd7521b3490/fendo-16-1516849-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/eb4f/12004233/d0552b27127b/fendo-16-1516849-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/eb4f/12004233/c30a2dfce1f9/fendo-16-1516849-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/eb4f/12004233/c27f58051960/fendo-16-1516849-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/eb4f/12004233/8c6b0c92305f/fendo-16-1516849-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/eb4f/12004233/acd7521b3490/fendo-16-1516849-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/eb4f/12004233/d0552b27127b/fendo-16-1516849-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/eb4f/12004233/c30a2dfce1f9/fendo-16-1516849-g005.jpg

相似文献

1
Involvement of the ovarian-specific Mro-IR in oogonia differentiation and oocyte development in freshwater giant prawn .卵巢特异性Mro-IR在淡水大虾卵原细胞分化和卵母细胞发育中的作用
Front Endocrinol (Lausanne). 2025 Apr 3;16:1516849. doi: 10.3389/fendo.2025.1516849. eCollection 2025.
2
Variation of prostaglandin E2 concentrations in ovaries and its effects on ovarian maturation and oocyte proliferation in the giant fresh water prawn, Macrobrachium rosenbergii.罗氏沼虾卵巢中前列腺素E2浓度的变化及其对卵巢成熟和卵母细胞增殖的影响
Gen Comp Endocrinol. 2015 Nov 1;223:129-38. doi: 10.1016/j.ygcen.2015.04.019. Epub 2015 May 8.
3
Function analysis and molecular characterization of cyclin A in ovary development of oriental river prawn, Macrobrachium nipponense.细胞周期蛋白 A 在日本沼虾卵巢发育中的功能分析和分子特征。
Gene. 2021 Jul 1;788:145583. doi: 10.1016/j.gene.2021.145583. Epub 2021 Mar 20.
4
Full Functional Sex Reversal Achieved Through Silencing of Gene in : Production of All-Male Monosex Freshwater Prawn.通过基因沉默实现完全功能性性别逆转：生产全雄单性淡水虾。
Front Endocrinol (Lausanne). 2022 Mar 17;12:772498. doi: 10.3389/fendo.2021.772498. eCollection 2021.
5
Oogenesis and ovarian development in the freshwater Crab Sodhiana iranica (Decapoda: Gecarcinuaidae) from the south of Iran.伊朗南部淡水蟹伊朗索氏蟹（十足目：地蟹科）的卵子发生和卵巢发育
Tissue Cell. 2015 Apr;47(2):213-20. doi: 10.1016/j.tice.2014.11.006. Epub 2014 Dec 18.
6
Effect of cadmium on the ovarian development in the freshwater prawn Macrobrachium rosenbergii (De Man).镉对罗氏沼虾（Macrobrachium rosenbergii）卵巢发育的影响。
Ecotoxicol Environ Saf. 2011 May;74(4):623-9. doi: 10.1016/j.ecoenv.2010.08.027. Epub 2011 Feb 5.
7
RNA interference shows that Spook, the precursor gene of 20-hydroxyecdysone (20E), regulates the molting of Macrobrachium nipponense.RNA 干扰表明，Spook（20-羟基蜕皮酮（20E）的前体基因）调节日本沼虾的蜕皮。
J Steroid Biochem Mol Biol. 2021 Oct;213:105976. doi: 10.1016/j.jsbmb.2021.105976. Epub 2021 Aug 18.
8
Insight into the Regulatory Relationships between the Insulin-Like Androgenic Gland Hormone Gene and the Insulin-Like Androgenic Gland Hormone-binding Protein Gene in Giant Freshwater Prawns ().洞悉巨型淡水虾（）中胰岛素样雄激素腺激素基因和胰岛素样雄激素腺激素结合蛋白基因之间的调控关系。
Int J Mol Sci. 2020 Jun 12;21(12):4207. doi: 10.3390/ijms21124207.
9
Molecular characterization of ovary-specific gene Mrfem-1 and siRNA-mediated regulation on targeting Mrfem-1 in the giant freshwater prawn, Macrobrachium rosenbergii.罗氏沼虾卵巢特异性基因Mrfem-1的分子特征及siRNA介导的针对Mrfem-1的调控
Gene. 2020 Sep 5;754:144891. doi: 10.1016/j.gene.2020.144891. Epub 2020 Jun 11.
10
Molecular cloning and characterization of cortical rod protein in the giant freshwater prawn Macrobrachium rosenbergii, a species not forming cortical rod structures in the oocytes.罗氏沼虾（一种在卵母细胞中不形成皮质杆状结构的物种）皮质杆蛋白的分子克隆与特性分析
Comp Biochem Physiol B Biochem Mol Biol. 2007 Oct;148(2):184-91. doi: 10.1016/j.cbpb.2007.05.008. Epub 2007 May 29.

本文引用的文献

1
Full Functional Sex Reversal Achieved Through Silencing of Gene in : Production of All-Male Monosex Freshwater Prawn.通过基因沉默实现完全功能性性别逆转：生产全雄单性淡水虾。
Front Endocrinol (Lausanne). 2022 Mar 17;12:772498. doi: 10.3389/fendo.2021.772498. eCollection 2021.
2
An overview of the insulin signaling pathway in model organisms Drosophila melanogaster and Caenorhabditis elegans.模式生物果蝇和秀丽隐杆线虫胰岛素信号通路概述。
Peptides. 2021 Nov;145:170640. doi: 10.1016/j.peptides.2021.170640. Epub 2021 Aug 24.
3
Heat Shock Proteins and Their Role in Pregnancy: Redefining the Function of "Old Rum in a New Bottle".
热休克蛋白及其在妊娠中的作用：重新定义“旧瓶装新酒”的功能
Front Cell Dev Biol. 2021 Apr 29;9:648463. doi: 10.3389/fcell.2021.648463. eCollection 2021.
4
The Roles of Insulin-Like Growth Factor Binding Protein Family in Development and Diseases.胰岛素样生长因子结合蛋白家族在发育和疾病中的作用。
Adv Ther. 2021 Feb;38(2):885-903. doi: 10.1007/s12325-020-01581-x. Epub 2020 Dec 17.
5
The "IAG-Switch"-A Key Controlling Element in Decapod Crustacean Sex Differentiation.“IA 开关”-十足目甲壳动物性别分化的关键控制元件。
Front Endocrinol (Lausanne). 2020 Sep 10;11:651. doi: 10.3389/fendo.2020.00651. eCollection 2020.
6
Functional analysis of ecdysteroid biosynthetic enzymes of the rice planthopper, Nilaparvata lugens.稻褐飞虱蜕皮激素生物合成酶的功能分析。
Insect Biochem Mol Biol. 2020 Aug;123:103428. doi: 10.1016/j.ibmb.2020.103428. Epub 2020 Jun 14.
7
Insight into the Regulatory Relationships between the Insulin-Like Androgenic Gland Hormone Gene and the Insulin-Like Androgenic Gland Hormone-binding Protein Gene in Giant Freshwater Prawns ().洞悉巨型淡水虾（）中胰岛素样雄激素腺激素基因和胰岛素样雄激素腺激素结合蛋白基因之间的调控关系。
Int J Mol Sci. 2020 Jun 12;21(12):4207. doi: 10.3390/ijms21124207.
8
Molecular characterization of ovary-specific gene Mrfem-1 and siRNA-mediated regulation on targeting Mrfem-1 in the giant freshwater prawn, Macrobrachium rosenbergii.罗氏沼虾卵巢特异性基因Mrfem-1的分子特征及siRNA介导的针对Mrfem-1的调控
Gene. 2020 Sep 5;754:144891. doi: 10.1016/j.gene.2020.144891. Epub 2020 Jun 11.
9
Vitellogenin Receptor (VgR) Mediates Oocyte Maturation and Ovarian Development in the Pacific White Shrimp ().卵黄原蛋白受体（VgR）介导太平洋白对虾的卵母细胞成熟和卵巢发育（）。
Front Physiol. 2020 May 15;11:485. doi: 10.3389/fphys.2020.00485. eCollection 2020.
10
Hormonal control of the crustacean molting gland: Insights from transcriptomics and proteomics.激素对甲壳动物蜕皮腺的调控：来自转录组学和蛋白质组学的见解。
Gen Comp Endocrinol. 2020 Aug 1;294:113493. doi: 10.1016/j.ygcen.2020.113493. Epub 2020 Apr 24.