gene knockout influences recombinant xylanase expression by .

is a methylotrophic yeast harboring a tightly regulated alcohol oxidase promoter ( ), which is now widely used for recombinant protein production. During expression phase by methanol induction, a methanol metabolism organelle peroxisome enlarged and occupied 80 % of cell through peroxins functions of matrix protein import and organelle division. Using a expressing xylanase in this study, each of all 23 genes of , encoding peroxin, was knockout to influence the peroxisome size, leading to changes of physiological status and recombinant xylanase expression. It was observed that knockout reduced peroxisome size by 54.3 %, increased xylanase expression by 29 %, decreased apoptosis ratio by 70.6 %. Transcriptome analysis revealed that gene knockout decreased 18 other genes of all three steps of peroxisome propagation, biogenesis, matrix protein import, and peroxisome fission. gene knockout influenced expression of ribosomal subunit-related and protein transportation significantly based on gene function annotation and enrichment analysis. Additionally, Therefore, gene knockout promoted xylanase folding correctly via Sec63 complex, and PDI1 significantly. In a summary, gene knockout provided a novel strategy to enhance recombinant xylanase by .