Villanueva Joseph A, Restrepo Diego, Ramirez-Gordillo Daniel
Department of Neurology, University of Colorado Anschutz Medical Campus, Aurora, CO, USA.
Neuroscience Program, University of Colorado Anschutz Medical Campus, Aurora, CO, USA.
Methods Mol Biol. 2025;2915:179-187. doi: 10.1007/978-1-0716-4466-9_12.
The development of optogenetics introduced a method for dynamic control of neuronal function. This advancement has catalyzed substantial progress in the field of neuroscience by providing a method to precisely activate or inhibit cells with light, allowing researchers to probe the role of neural circuits in behavior and interactions between circuits in different regions of the brain. We have developed an inexpensive device to deliver light for closed-loop optogenetic stimulation of neurons at different phases of the theta local field potential (LFP). LFP data is acquired with an INTAN RHD2000 recording system. An analog LFP output from the INTAN board is filtered in the theta frequency and an Arduino UNO detects troughs and peaks and sends a TTL pulse to a laser which precisely controls light delivery at the desired theta phases. We use this system to deliver light stimulation at the peak or trough of theta, but users can edit the code for delivery at other phases. We have made the code open source, and it can be downloaded from https://github.com/restrepd/drgClosedLoop .
光遗传学的发展引入了一种动态控制神经元功能的方法。这一进展通过提供一种用光精确激活或抑制细胞的方法，推动了神经科学领域的重大进步，使研究人员能够探究神经回路在行为中的作用以及大脑不同区域回路之间的相互作用。我们开发了一种廉价的设备，用于在theta局部场电位（LFP）的不同阶段为神经元的闭环光遗传学刺激提供光。LFP数据通过INTAN RHD2000记录系统采集。来自INTAN板的模拟LFP输出在theta频率下进行滤波，Arduino UNO检测波谷和波峰，并向激光器发送一个TTL脉冲，该激光器精确控制在所需的theta相位进行光传输。我们使用这个系统在theta的峰值或波谷进行光刺激，但用户可以编辑代码以在其他相位进行传输。我们已将代码开源，可从https://github.com/restrepd/drgClosedLoop下载。
