Watanabe S, Yamamoto R, Ogata M, Murakami T
Acta Med Okayama. 1985 Jun;39(3):239-46. doi: 10.18926/AMO/31534.
Erythrocytes in human blood stored for 120 days were collected by centrifugation after dispersion in buffered physiological saline. The aged erythrocytes thus collected were incubated with inosine, adenine, glucose or other media, and their shapes and ATP levels were studied by scanning electron microscopy and a luciferine-luciferase method. The aged erythrocytes incubated in a mixture of adenine and inosine markedly regained their ATP levels, and also showed a marked transformation from spiked spherocytes to normal discocytes. Incubation with inosine alone restored ATP levels of the aged erythrocytes to some extent, but did not result in morphological rejuvenation. Incubation in a mixture of citrate and glucose caused morphological rejuvenation, though it restored ATP levels less effectively than incubation in inosine alone. Incubation with adenine alone neither restored ATP levels nor resulted in morphological rejuvenation of the stored erythrocytes.
将储存120天的人血红细胞分散于缓冲生理盐水中后，通过离心收集。将如此收集的衰老红细胞与肌苷、腺嘌呤、葡萄糖或其他培养基一起孵育，并用扫描电子显微镜和荧光素-荧光素酶法研究其形状和ATP水平。在腺嘌呤和肌苷混合物中孵育的衰老红细胞显著恢复了其ATP水平，并且还显示出从尖刺球形红细胞向正常盘状红细胞的显著转变。单独用肌苷孵育在一定程度上恢复了衰老红细胞的ATP水平，但未导致形态恢复。在柠檬酸盐和葡萄糖混合物中孵育导致形态恢复，尽管其恢复ATP水平的效果不如单独用肌苷孵育。单独用腺嘌呤孵育既未恢复ATP水平，也未导致储存红细胞的形态恢复。
