Nakao M, Hoshino K, Nakao T
J Bioenerg Biomembr. 1981 Dec;13(5-6):307-16. doi: 10.1007/BF00743208.
Erythrocytes in long-preserved blood are spherical, but when the cells are incubated with inosine and adenine, the resulting increase in ATP content is accompanied by a shape change of the cells to discoidal form via a crenated form. The cells incubated with adenine alone or with no addition remain almost unchanged in shape. When incubated with inosine alone, the elevation in ATP level is less than that with both inosine and adenine, and the cell shape remains unchanged or changes partially into a crenated form. These phenomena occur in the presence of EDTA as well as in the absence of serum protein in the media. The cell volumes are measured as packed cell volume after centrifugation, by means of a Coulter counter (model S), and by determination of the intercellular space by the use of 131I-labeled bovine serum albumin. The results show that no alteration in cell volume occurs during the shape changes. Accordingly, the surface area of the cell must increase with increase in the ATP content. This suggests that both the lipid bimolecular layer and the undermembrane structure are altered during the shape change.
长期保存血液中的红细胞呈球形，但当这些细胞与肌苷和腺嘌呤一起孵育时，由此导致的ATP含量增加伴随着细胞形状从有锯齿状转变为盘状。单独与腺嘌呤孵育或不添加任何物质的细胞形状几乎不变。仅与肌苷孵育时，ATP水平的升高低于同时与肌苷和腺嘌呤孵育时，细胞形状保持不变或部分转变为有锯齿状。这些现象在存在EDTA以及培养基中无血清蛋白的情况下都会发生。通过使用库尔特计数器（型号S）在离心后测量血细胞比容来测定细胞体积，并通过使用131I标记的牛血清白蛋白来测定细胞间空间。结果表明，在形状变化过程中细胞体积没有改变。因此，细胞表面积必须随着ATP含量的增加而增加。这表明在形状变化过程中脂质双分子层和膜下结构都发生了改变。
