Isaac Roger Derick, Sanjeev Kavitha, Subbulakshmi C L, Amirtharaj L Vijay, Sekar Mahalaxmi
Department of Conservative Dentistry and Endodontics, Best Dental Science College, Madurai, Tamil Nadu, India.
Department of Conservative Dentistry and Endodontics, SRM Dental College, SRM Institute of Science and Technology, Chennai, Tamil Nadu, India.
J Conserv Dent. 2022 May-Jun;25(3):297-305. doi: 10.4103/jcd.jcd_79_22. Epub 2022 Jun 13.
which comprise only 2% have long been presumed to be the specific pathogen responsible for caries. A novel caries associated bacterium namely is recognized to be ecologically competitive in active caries lesions. The actual pathogen needs to be identified, so as to specifically target and reduce the prevalence of caries in a given community.
The aim of the study is to evaluate the presence of in combination with other bacteria in caries risk adolescence.
Sixty adolescent subjects were screened. Phase I-to determine the prevalence of in saliva, plaque and dentinal caries samples of low and high caries risk individuals ( = 30 each) using polymerase chain reaction (PCR); Phase-II-to identify its presence by 16SrRNA metagenomic analysis and quantitatively evaluate the cariogenic pathogen using high-resolution melt curve analysis and real-time PCR.
Highest prevalence of was observed in dentinal caries followed by plaque and saliva samples of high caries risk individuals under PCR analysis. Metagenomic analysis showed two-fold statistically increased presence of and () in dentinal samples compared to plaque samples ( = 0.05). recorded the minimum.
is identified as one of the predominant microorganism.
长期以来，仅占2%的[具体细菌名称未给出]一直被认为是导致龋齿的特定病原体。一种新的与龋齿相关的细菌，即[细菌名称未给出]，被认为在活跃的龋齿病变中具有生态竞争力。需要确定实际的病原体，以便在特定社区中针对性地降低龋齿的患病率。
本研究的目的是评估[细菌名称未给出]与其他细菌在龋齿风险青少年中的存在情况。
对60名青少年受试者进行筛查。第一阶段——使用聚合酶链反应（PCR）确定低龋齿风险和高龋齿风险个体（各30名）的唾液、牙菌斑和牙本质龋样本中[细菌名称未给出]的患病率；第二阶段——通过16SrRNA宏基因组分析确定其存在情况，并使用高分辨率熔解曲线分析和实时PCR定量评估致龋病原体。
在PCR分析中，高龋齿风险个体的牙本质龋中观察到[细菌名称未给出]的患病率最高，其次是牙菌斑和唾液样本。宏基因组分析显示，与牙菌斑样本相比，牙本质样本中[细菌名称未给出]和[另一细菌名称未给出]（[具体细菌名称未给出]）的存在在统计学上增加了两倍（P = 0.05）。[细菌名称未给出]的含量最低。
[细菌名称未给出]被确定为主要微生物之一。
