Marsolier Justine, Moutaux Eve, Grosselin Kevin, Griffiths Andrew, Vallot Céline
CNRS UMR3244, Institut Curie, PSL University, Paris, France.
Translational Research Department, Institut Curie, PSL University, Paris, France.
Methods Mol Biol. 2025;2919:213-239. doi: 10.1007/978-1-0716-4486-7_12.
Our high-throughput scChIP-seq approach combines droplet microfluidics with single-cell DNA barcoding to study the heterogeneity of epigenomes (H3K27me3, H3K4me3, H3K27Ac, H3K4me1) in a cellular population of several thousand cells with a coverage of up to 10,000 unique loci per cell.
我们的高通量单细胞染色质免疫沉淀测序（scChIP-seq）方法将微滴微流控技术与单细胞DNA条形码技术相结合，以研究数千个细胞群体中表观基因组（H3K27me3、H3K4me3、H3K27Ac、H3K4me1）的异质性，每个细胞的覆盖范围高达10000个独特位点。
