利用高通量液滴单细胞染色质免疫沉淀测序进行单细胞表观基因组分析
Single-cell Epigenomic Profiling with High-throughput Droplet scChIP-seq.
作者信息
Marsolier Justine, Moutaux Eve, Grosselin Kevin, Griffiths Andrew, Vallot Céline
机构信息
CNRS UMR3244, Institut Curie, PSL University, Paris, France.
Translational Research Department, Institut Curie, PSL University, Paris, France.
出版信息
Methods Mol Biol. 2025;2919:213-239. doi: 10.1007/978-1-0716-4486-7_12.
PMID:40257565
Abstract
Our high-throughput scChIP-seq approach combines droplet microfluidics with single-cell DNA barcoding to study the heterogeneity of epigenomes (H3K27me3, H3K4me3, H3K27Ac, H3K4me1) in a cellular population of several thousand cells with a coverage of up to 10,000 unique loci per cell.
摘要
我们的高通量单细胞染色质免疫沉淀测序（scChIP-seq）方法将微滴微流控技术与单细胞DNA条形码技术相结合，以研究数千个细胞群体中表观基因组（H3K27me3、H3K4me3、H3K27Ac、H3K4me1）的异质性，每个细胞的覆盖范围高达10000个独特位点。
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