Nikzad-Chaleshtori Maedeh, Asgari Mohsen, Rezaeizadeh Golnoosh, Aali Faranak, Doosti Abbas
Department of Biology, Shahrekord Branch, Islamic Azad University, Shahrekord, Iran.
Biotechnology Research Center, Shahrekord Branch, Islamic Azad University, Shahrekord, Iran.
Immunol Res. 2025 Apr 22;73(1):74. doi: 10.1007/s12026-025-09625-6.
There is a strong association between Helicobacter pylori (H. pylori) and the occurrence of gastritis and gastric mucosal lymphoma in the human population. Vaccination is a viable preventive measure in light of the escalating issue of antibiotic resistance. The use of DNA vaccines presents a potentially effective approach. This study used the utilization of antigenic H. pylori urease E subunit (UreE) for the development of a DNA vaccine. The UreE gene was chemically cloned into pIRES2-DsRed-Express (pDNA), and PCR and restriction enzyme digestion verified the cloning. The immunogenicity and immune-protective efficacy of the vaccination were assessed in BALB/c mice. In contrast, blood samples from BALB/c mice inoculated with pDNA-UreE showed higher levels of IgG, IFN-γ, IL- 4, and IL- 17. Furthermore, stomach damage and bacterial loads were reduced, and BALB/c mice inoculated with pDNA-UreE exhibited a significant protection rate (87.5%) against the H. pylori challenge. pDNA-UreE generated a combination of Th1-Th2-Th17 immune responses, perhaps contributing to adequate protection. Based on our findings, using this DNA immunization as a preventive measure against H. pylori infection is a viable approach.
幽门螺杆菌（H. pylori）与人类胃炎及胃黏膜淋巴瘤的发生之间存在密切关联。鉴于抗生素耐药性问题不断升级，疫苗接种是一种可行的预防措施。使用DNA疫苗是一种潜在的有效方法。本研究利用幽门螺杆菌抗原性脲酶E亚基（UreE）开发一种DNA疫苗。将UreE基因化学克隆到pIRES2-DsRed-Express（pDNA）中，通过PCR和限制性内切酶消化验证克隆情况。在BALB/c小鼠中评估该疫苗接种的免疫原性和免疫保护效力。相比之下，接种pDNA-UreE的BALB/c小鼠血液样本中IgG、IFN-γ、IL-4和IL-17水平更高。此外，胃损伤和细菌载量降低，接种pDNA-UreE的BALB/c小鼠对幽门螺杆菌攻击表现出显著的保护率（87.5%）。pDNA-UreE产生了Th1-Th2-Th17免疫反应的组合，这可能有助于提供充分的保护。基于我们的研究结果，将这种DNA免疫作为预防幽门螺杆菌感染的措施是一种可行的方法。
