Thieme-Ehlert Marie-Theres, Jacobs Thomas, Brandi Johannes, Mackroth Maria Sophia
Division of Infectious Diseases, I. Medical Department, University Medical Center Hamburg Eppendorf, Hamburg, Germany.
Protozoa Immunology, Bernhard Nocht Institut for Tropical Medicine, Hamburg, Germany.
Cytometry A. 2025 Jun;107(6):364-371. doi: 10.1002/cyto.a.24937. Epub 2025 Apr 22.
This 36-color flow cytometry panel is designed to characterize multiple lymphocyte compartments, with a focus on T cells, their memory subpopulations, and immune checkpoints in human whole blood samples. In clinical settings, the amount of blood available from patients for scientific research is often limited. This restriction may be further exacerbated when working with samples from small children or in resource-poor settings-both scenarios commonly encountered in malaria and infectious disease research. Accordingly, this panel is designed to maximize the information that can be obtained from as little as 200 μL whole blood using flow cytometry. This panel allows a phenotypic characterization of the main subpopulations within T cells, as well as B cells and NK cells. It includes markers for the analysis of memory subpopulations, regulatory T cell subsets, and T follicular helper cells. Furthermore, surface and intracellular markers for activation and differentiation, effector functions, exhaustion, and immune checkpoints are included, allowing detailed characterization of the main lymphocyte subsets, in particular T cells. This panel was optimized for the analysis of fresh human blood samples obtained from malaria patients, but it may be adapted to the analysis of isolated PBMC or tissue samples, as well as samples from patients with other infectious or inflammatory diseases.
这个36色流式细胞术检测板旨在对多个淋巴细胞亚群进行表征，重点关注人类全血样本中的T细胞、其记忆亚群和免疫检查点。在临床环境中，患者可用于科研的血量通常有限。当处理来自幼儿的样本或在资源匮乏的环境中时，这种限制可能会进一步加剧——这两种情况在疟疾和传染病研究中都很常见。因此，该检测板旨在利用流式细胞术从低至200 μL全血中获取最多的信息。该检测板能够对T细胞以及B细胞和NK细胞内的主要亚群进行表型表征。它包括用于分析记忆亚群、调节性T细胞亚群和T滤泡辅助细胞的标志物。此外，还包括用于激活和分化、效应功能、耗竭和免疫检查点的表面和细胞内标志物，从而能够对主要淋巴细胞亚群，特别是T细胞进行详细表征。该检测板针对从疟疾患者获得的新鲜人类血液样本的分析进行了优化，但也可适用于分离的外周血单核细胞或组织样本，以及来自患有其他感染性或炎症性疾病患者的样本。
