Liu Yi-Xian, Jia Bao-Zhu, Zhang Xu-Chao, Hou Fei, Liu Jun-Peng, Guo Wei-Yun, Meng Yuan, Xu Zhen-Lin, Luo Lin
Guangdong Provincial Key Laboratory of Food Quality and Safety, College of Food Science, South China Agricultural University, Guangzhou, 510642, China.
College of Biology and Food Engineering, Guangdong University of Education, Guangzhou, 510303, China.
Mikrochim Acta. 2025 Apr 22;192(5):316. doi: 10.1007/s00604-025-07175-0.
Chronic dietary intake of 17β-estradiol (E) poses significant health risks, necessitating rapid and accurate analytical techniques for effective surveillance. This study presents a magnetic particle (MP)-assisted non-competitive chemiluminescent immunoassay (NCLIA) designed for E quantification in milk. The NCLIA employs anti-E monoclonal antibody (mAb)-coated MPs to capture E target molecules, followed by the recognition of acridinium ester (AE)-conjugated anti-E@mAb immunocomplex antibody. Magnetic separation facilitates efficient isolation of immunocomplexes before automated luminescence measurement. This NCLIA demonstrated superior performance, without the need for complex pretreatment of milk samples, significantly simplifying the analysis process. The detection limit was as low as 1.57 pg/mL, and total detection time was 8 min. Additionally, NCLIA's results were highly consistent with those obtained by liquid chromatography-tandem mass spectrometry (LC-MS/MS), validating their reliability in analyzing real samples. Therefore, this study presents an efficient and reliable tool for the rapid screening and detection of 17β-estradiol in milk, which holds significant value for food safety monitoring.
长期通过饮食摄入17β-雌二醇（E）会带来重大健康风险，因此需要快速准确的分析技术进行有效监测。本研究提出了一种用于牛奶中E定量的磁颗粒（MP）辅助非竞争性化学发光免疫分析（NCLIA）方法。该NCLIA采用包被有抗E单克隆抗体（mAb）的MP来捕获E靶分子，随后识别吖啶酯（AE）偶联的抗E@mAb免疫复合物抗体。磁分离有助于在自动发光测量前高效分离免疫复合物。这种NCLIA表现出卓越的性能，无需对牛奶样品进行复杂的预处理，显著简化了分析过程。检测限低至1.57 pg/mL，总检测时间为8分钟。此外，NCLIA的结果与液相色谱-串联质谱（LC-MS/MS）获得的结果高度一致，验证了其在分析实际样品中的可靠性。因此，本研究提出了一种用于牛奶中17β-雌二醇快速筛查和检测的高效可靠工具，对食品安全监测具有重要价值。
