Liu Chao, Li Jiapeng, Yan Lihong, Zhu Yuting, Li Zikun, Cao Chengquan, Wang Yiping
College of Forestry and Biotechnology, Zhejiang Agricultural and Forestry University, Lin'an 311300, China.
School of Management, Chengdu University of Information Technology, Chengdu 610225, China.
Insects. 2025 Mar 18;16(3):316. doi: 10.3390/insects16030316.
Within the context of global warming, understanding the molecular mechanisms behind physiological plasticity and local adaptation is essential for insect populations. This study performed an integrated miRNA and mRNA analysis on larvae exposed to temperatures of 20 °C, 24 °C, 28 °C, and 32 °C. Under varying thermal conditions, 1983 genes exhibited differential expression (i.e., DEGs). These genes showed significant enrichment in Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways related to carbohydrate metabolism, glycan biosynthesis and metabolism. Notably, we detected that the "neuroactive ligand-receptor interaction" signaling pathway, which is involved in environmental information processing, was significantly upregulated in the 28 °C and 32 °C treatment groups. This indicates that starting at 28 °C, needs to maintain normal cellular physiological functions by regulating ligand-receptor binding and signal transduction. Furthermore, 220 differentially expressed miRNAs (DEMs) were detected under the different temperature treatment conditions. An interaction network was constructed between key DEMs and DEGs, revealing 12 significant DEM-DEG regulatory pairs in under different temperature treatments. We found three miRNA-mRNA candidate modules that could be involved in 's response to high temperature, including ggo-miR-1260b and ptr-miR-1260b/RN001_010114, CM069438.1_43851/RN001_014852, and CM069438.1_43851/RN001_014877. Our data provide deeper insights into the molecular responses of to the high temperature at the miRNA and mRNA levels.
在全球变暖的背景下，了解生理可塑性和局部适应性背后的分子机制对昆虫种群至关重要。本研究对暴露于20°C、24°C、28°C和32°C温度下的幼虫进行了miRNA和mRNA的综合分析。在不同的热条件下，1983个基因表现出差异表达（即DEGs）。这些基因在与碳水化合物代谢、聚糖生物合成和代谢相关的京都基因与基因组百科全书（KEGG）通路中显著富集。值得注意的是，我们检测到参与环境信息处理的“神经活性配体-受体相互作用”信号通路在28°C和32°C处理组中显著上调。这表明从28°C开始，[昆虫名称]需要通过调节配体-受体结合和信号转导来维持正常的细胞生理功能。此外，在不同温度处理条件下检测到220个差异表达的miRNA（DEMs）。构建了关键DEM和DEG之间的相互作用网络，揭示了在不同温度处理下[昆虫名称]中有12个显著的DEM-DEG调控对。我们发现了三个可能参与[昆虫名称]对高温反应的miRNA-mRNA候选模块，包括ggo-miR-1260b和ptr-miR-1260b/RN001_010114、CM069438.1_43851/RN001_014852以及CM069438.1_43851/RN001_014877。我们的数据为[昆虫名称]在miRNA和mRNA水平上对高温的分子反应提供了更深入的见解。
