Cauquil Marie, Olivry Thierry
Nextmune, 3 rue Pierre-Gilles de Gennes, ZA Le Causse, 81100 Castres, France.
Nextmune AB, Riddargatan 19, 114 57 Stockholm, Sweden.
Vet Sci. 2025 Mar 2;12(3):226. doi: 10.3390/vetsci12030226.
Canine atopic dermatitis (AD) is a T-cell-driven inflammatory skin disease, characterized by an imbalance between the Th1 and Th2 immune responses. Probiotics (live bacteria) and postbiotics (inactivated, killed bacteria) have garnered attention for the management of AD in humans and dogs. Both probiotics and postbiotics possess immunomodulating properties that could be beneficial for allergic patients. This study aims to evaluate the immunomodulating effects of Tyndallized (heat-killed) postbiotics of and which are active components of the Linkskin products (Nextmune, Palazzo Pignano, Cremona, Italy). Peripheral blood mononuclear cells (PBMCs) were isolated from healthy dogs and incubated separately with each postbiotic. The cytokine levels in the supernatants were measured before and after 12, 24, 48, and 72 h of incubation. Both Tyndallized lactobacilli significantly increased the levels of IL-12 and IFN-γ (Th1 cytokines) and IL-10 (associated with T regulatory cells), while the levels of the Th2 cytokine IL-4 remained stable. Overall, these two postbiotics stimulated canine PBMCs to produce a cytokine profile typically associated with an anti-allergic response. Further studies are needed to evaluate the benefit of these postbiotics as an adjuvant for the reactive treatment or for the prevention of relapses of allergic flares in atopic dogs.
犬特应性皮炎（AD）是一种由T细胞驱动的炎症性皮肤病，其特征在于Th1和Th2免疫反应之间的失衡。益生菌（活细菌）和后生元（灭活、杀死的细菌）在人类和犬类AD的管理方面受到了关注。益生菌和后生元都具有免疫调节特性，这可能对过敏患者有益。本研究旨在评估Linkskin产品（Nextmune，意大利克雷莫纳市皮尼亚诺宫）的活性成分——热灭活后生元的免疫调节作用。从健康犬只中分离出外周血单核细胞（PBMCs），并分别与每种后生元一起孵育。在孵育12、24、48和72小时之前和之后测量上清液中的细胞因子水平。两种热灭活乳酸杆菌均显著提高了IL-12、IFN-γ（Th1细胞因子）和IL-10（与调节性T细胞相关）的水平，而Th2细胞因子IL-4的水平保持稳定。总体而言，这两种后生元刺激犬PBMCs产生通常与抗过敏反应相关的细胞因子谱。需要进一步研究来评估这些后生元作为特应性犬反应性治疗辅助剂或预防过敏发作复发的益处。
