Sheikhi Abdolkarim, Giti Hojjat, Heibor Mohammad Reza, Jafarzadeh Abdollah, Shakerian Mansour, Baharifar Narges, Niruzad Fereidon, Moghaddam Ali Sadeghi, Kokhaei Parviz, Baghaeifar Mohammad
Dpartment of Immunology, Faculty of Medicine, Dezful University of Medical Sciences, Dezful, Iran.
Cellular and Molecular Immunology Research laboratory, Faculty of Medicine, Dezful University of Medical Sciences, Dezful, Iran.
Drug Res (Stuttg). 2017 Dec;67(12):724-729. doi: 10.1055/s-0043-117612. Epub 2017 Sep 12.
Atopic dermatitis (AD) is an inflammatory skin disease which may be due to the imbalance between Th1-, Th2 and Treg cell-related immune responses. Evidences suggest that appropriate stimulation with probiotics may correct the skewed immune response in children with AD. The aim was to determine the effects of the yogurt culture on the secretion of Th1/Th2/Treg type cytokines by PBMCs from children with AD. was cultivated on MRS broth. The PBMCs from 20 children with AD were separated by Ficoll-Hypaque centrifugation and co-cultured with different concentrations of UV killed bacteria in RPMI-1640 plus 10% FCS for 48/72 h. The levels of IL-10, IL-4, IL-12 and IFN-γ were measured in supernatant of PBMCs by ELISA. significantly up-regulated the secretion of IL-10, IL-12 and IFN-γ, whereas decreased the secretion of IL-4 by PBMCs at both incubation times 48 h/72 h and both bacteria:PBMCs ratios 100:1/50:1, compared to control (p<0.05). There were no significant differences between incubation times 48 h and 72 h regarding the secretion levels of IL-12, IFN-γ and IL-4. However, the secretion of IL-10 by -stimulated PBMCs at incubation time 72 h and in the presence of bacteria:PBMCs ratio 100:1 was significantly higher than in incubation time 48 h and in the presence of bacteria:PBMCs ratio 50:1 (P<0.000 and P<0.00, respectively). These data show that may modulate the secretion of Th1-, Th2-Treg-related cytokines in AD patients. Therefore, the possible potential therapeutic of for treatment of AD should be consider in further investigation.
特应性皮炎（AD）是一种炎症性皮肤病，可能是由于Th1、Th2和调节性T细胞（Treg）相关免疫反应之间的失衡所致。有证据表明，用益生菌进行适当刺激可能会纠正AD患儿失衡的免疫反应。本研究旨在确定酸奶培养物对AD患儿外周血单核细胞（PBMC）分泌Th1/Th2/Treg型细胞因子的影响。在MRS肉汤中培养。通过Ficoll-Hypaque离心法分离20例AD患儿的PBMC，并在含有10%胎牛血清（FCS）的RPMI-1640培养基中与不同浓度的紫外线灭活细菌共培养48/72小时。采用酶联免疫吸附测定（ELISA）法检测PBMC上清液中白细胞介素-10（IL-10）、白细胞介素-4（IL-4）、白细胞介素-12（IL-12）和干扰素-γ（IFN-γ）的水平。与对照组相比，在48小时/72小时的孵育时间以及100:1/50:1的两种细菌与PBMC比例下，[细菌名称]均显著上调了PBMC中IL-10、IL-12和IFN-γ的分泌，而降低了IL-4的分泌（p<0.05）。在IL-12、IFN-γ和IL-4的分泌水平方面，48小时和72小时的孵育时间之间没有显著差异。然而，在72小时孵育时间且细菌与PBMC比例为100:1的情况下，[细菌名称]刺激的PBMC分泌的IL-10显著高于48小时孵育时间且细菌与PBMC比例为50:1的情况（分别为P<0.000和P<0.00）。这些数据表明，[细菌名称]可能调节AD患者中Th1、Th2-Treg相关细胞因子的分泌。因此，在进一步研究中应考虑[细菌名称]治疗AD的潜在可能性。
