Auer G U, Bäckdahl M, Forsslund G M, Askensten U G
Anal Quant Cytol Histol. 1985 Jun;7(2):97-106.
DNA levels in nonneoplastic and neoplastic human thyroid cells were studied by slide-cytophotometric and flow-cytophotometric techniques. Normal epithelial thyroid cells and cells from nonneoplastic thyroid disorders, i.e., toxic goiter, colloid goiter and chronic lymphocytic thyroiditis, had euploid (diploid, polyploid) DNA levels. The same was the case for benign tumors. Malignant tumors had either euploid DNA levels, indistinguishable from those in nonneoplastic and benign neoplastic cell populations, or aneuploid DNA levels. Taking into account the histopathologic diagnosis, clinical course and DNA level, the results indicate that DNA measurements in thyroid gland lesions are of limited diagnostic help but may contribute valuable prognostic information. To obtain representative and accurate DNA measurements, combined slide-cytophotometric and flow-cytophotometric techniques should be used.
采用玻片细胞光度法和流式细胞光度法研究了非肿瘤性和肿瘤性人甲状腺细胞中的DNA水平。正常甲状腺上皮细胞以及来自非肿瘤性甲状腺疾病（即毒性甲状腺肿、胶样甲状腺肿和慢性淋巴细胞性甲状腺炎）的细胞具有整倍体（二倍体、多倍体）DNA水平。良性肿瘤的情况也是如此。恶性肿瘤要么具有与非肿瘤性和良性肿瘤细胞群体中无法区分的整倍体DNA水平，要么具有非整倍体DNA水平。综合组织病理学诊断、临床病程和DNA水平来看，结果表明甲状腺病变中的DNA测量在诊断方面帮助有限，但可能提供有价值的预后信息。为了获得具有代表性和准确的DNA测量结果，应采用玻片细胞光度法和流式细胞光度法相结合的技术。
