Van Dessel G A, Lagrou A R, Hilderson H J, Dierick W S, Lauwers W F
J Biol Chem. 1979 Sep 25;254(18):9305-10.
After preparative isolation, the carbohydrate, long chain base, and fatty acid composition of the major gangliosides from bovine thyroid have been analyzed. The structures were elucidated by determining the molar ratio of the building blocks, permethylation analysis, and enzymatic degradation studies. The following structures are identified: N-Acetylneuraminyl(2,3)-galactosyl(1,4)glucosyl(1,1)ceramidie; N-glycolyneuraminyl(2,3)galactosyl(1,4)glucosyl(1,1)ceramide; galactosyl(1,3)N-acetylgalactosaminyl(3,2)N-acetylneuraminylgalactosyl(1,4)glucosyl(1,1)ceramide; fucosyl(1,2)galactosyl(1,3)N-acetylgalactosaminyl(3,2)N-acetylneuraminylgalactosyl(1,4)glucosyl(1,1)-ceramide. The structures were confirmed by direct inlet mass spectrometry of the permethylated gangliosides and the corresponding asialo derivatives. Structures are proposed for common ions in the different mass spectra.
经过制备性分离后，对来自牛甲状腺的主要神经节苷脂的碳水化合物、长链碱基和脂肪酸组成进行了分析。通过确定结构单元的摩尔比、全甲基化分析和酶促降解研究阐明了其结构。鉴定出以下结构：N-乙酰神经氨酸基（2,3）-半乳糖基（1,4）葡萄糖基（1,1）神经酰胺；N-糖基神经氨酸基（2,3）半乳糖基（1,4）葡萄糖基（1,1）神经酰胺；半乳糖基（1,3）N-乙酰半乳糖胺基[（3,2）N-乙酰神经氨酸基]（1,4）半乳糖基（1,4）葡萄糖基（1,1）神经酰胺；岩藻糖基（1,2）半乳糖基（1,3）N-乙酰半乳糖胺基[（3,2）N-乙酰神经氨酸基]（1,4）半乳糖基（1,4）葡萄糖基（1,1）神经酰胺。通过对全甲基化神经节苷脂和相应的去唾液酸衍生物进行直接进样质谱分析，证实了这些结构。还针对不同质谱中的常见离子提出了结构。
