Ferrero D, Gabbianelli M, Peschle C, Lange B, Rovera G
Blood. 1985 Sep;66(3):496-502.
A panel of ten monoclonal antibodies which react with antigens present on the surface of myeloid leukemic cells was used to investigate the distribution of these antigens on normal hemopoietic stem cells and progenitor cells at various stages of maturity. A population of immature cells, possibly stem cells, that are capable of regenerating CFU-GM in long-term marrow cultures reacts with four antibodies recognizing antigens abundantly expressed in leukemic cells, but does not react with antibodies against Ia-like molecules or against carbohydrate determinants specific for myeloid cells. Progenitor cells that form mixed colonies in semisolid medium (CFU-GEMM), early erythroid (BFU-E) and early myelomonocytic (type 1 CFU-GM) progenitors retain the antigens present on the hypothetical stem cell population and begin to express Ia-like antigens. As they differentiate, myeloid and erythroid progenitors undergo a series of quantitative and qualitative shifts in surface phenotype. They begin to express stage-related, lineage-specific antigens and cease expressing antigens common to early cells of different lineages. The identification of antigens present on very immature normal progenitor cells should be valuable in future studies aimed at the detailed characterization of this relatively little-known hemopoietic cell population.
使用一组与髓系白血病细胞表面存在的抗原发生反应的十种单克隆抗体，来研究这些抗原在正常造血干细胞和不同成熟阶段祖细胞上的分布。在长期骨髓培养中能够再生CFU-GM的一群未成熟细胞，可能是干细胞，与识别白血病细胞中大量表达的抗原的四种抗体发生反应，但不与针对Ia样分子或髓系细胞特异性碳水化合物决定簇的抗体发生反应。在半固体培养基中形成混合集落的祖细胞（CFU-GEMM）、早期红系祖细胞（BFU-E）和早期髓单核祖细胞（1型CFU-GM）保留了假设干细胞群体上存在的抗原，并开始表达Ia样抗原。随着它们的分化，髓系和红系祖细胞在表面表型上经历一系列数量和质量上的变化。它们开始表达阶段相关的、谱系特异性抗原，并停止表达不同谱系早期细胞共有的抗原。鉴定非常未成熟的正常祖细胞上存在的抗原，对于未来旨在详细表征这一相对鲜为人知的造血细胞群体的研究应该是有价值的。
