Bian Yihao, Fu Kunming, Xu Ruotong, Guan Teng, Huo Aotong, Zhang Ruibao, Li Xueqin, Qiu Fuguo, Zhang Yongji
State Key Laboratory of Pollution Control and Resource Reuse, College of Environmental Science and Engineering, Tongji University, Shanghai, 200092, China; Shanghai Institute of Pollution Control and Ecological Security, Shanghai, 200092, China; Key Laboratory of Urban Storm Water System and Water Environment Ministry of Education, Beijing University of Civil Engineering and Architecture, Beijing, 100044, China.
Key Laboratory of Urban Storm Water System and Water Environment Ministry of Education, Beijing University of Civil Engineering and Architecture, Beijing, 100044, China.
Environ Res. 2025 Aug 1;278:121630. doi: 10.1016/j.envres.2025.121630. Epub 2025 Apr 22.
The simultaneous partial nitrification, anammox, and denitrification (SNAD) process is widely applied for treating high-ammonia wastewater, but its application to low-ammonia organic wastewater has been scarcely explored. In this study, a partial nitrification and denitrification coupled with simultaneous partial nitrification, anammox, and denitrification (PND-SNAD) system was established to treat organic wastewater with low ammonia concentration. Experimental results revealed that sulfide at 5 mg/L selectively inhibited nitrite-oxidizing bacteria (NOB) but had little effect on ammonium-oxidizing bacteria (AOB). Finally, NOB was suppressed in PND system by intermittently adding sulfide to the PND system. The PND system provided nitrite and activated sludge enriched with AOB to the SNAD system during stable operation. The SNAD system demonstrated chemical oxygen demand (COD) and nitrogen removal efficiencies of 89.86 % and 86.45 %. Candidatus Brocadia and Nitrosomonas were the main ammonium oxidizing bacteria (AnAOB) and AOB. The contribution of AOB and denitrifying bacteria (DNB) to nitrogen transformation was 67.15 % and 25.33 % in the PND system. In the SNAD system, the contributions of AnAOB, AOB, and DNB were 34.40 %, 33.59 %, and 27.56 %, respectively. Overall, this study provided a new sustainable strategy for treating organic wastewater with low ammonia concentration.
同步短程硝化、厌氧氨氧化和反硝化（SNAD）工艺被广泛应用于处理高氨废水，但其在低氨有机废水处理中的应用鲜有研究。本研究建立了一种部分硝化与反硝化耦合同步短程硝化、厌氧氨氧化和反硝化（PND-SNAD）系统来处理低氨浓度的有机废水。实验结果表明，5mg/L的硫化物能选择性抑制亚硝酸盐氧化细菌（NOB），但对氨氧化细菌（AOB）影响较小。最后，通过向PND系统间歇添加硫化物，抑制了PND系统中的NOB。在稳定运行期间，PND系统为SNAD系统提供了亚硝酸盐和富含AOB的活性污泥。SNAD系统的化学需氧量（COD）和氮去除效率分别为89.86%和86.45%。“Candidatus Brocadia”和亚硝化单胞菌是主要的厌氧氨氧化细菌（AnAOB）和AOB。在PND系统中，AOB和反硝化细菌（DNB）对氮转化的贡献分别为67.15%和25.33%。在SNAD系统中，AnAOB、AOB和DNB的贡献分别为34.40%、33.59%和27.56%。总体而言，本研究为处理低氨浓度的有机废水提供了一种新的可持续策略。
