Alzahrani Abdulhakeem
Department of Food Science & Nutrition, College of Food and Agricultural Sciences, King Saud University, Riyadh, Saudi Arabia.
NPJ Sci Food. 2025 Apr 24;9(1):59. doi: 10.1038/s41538-025-00422-x.
Bacteria-assisted click chemistry for enhanced nanozyme-based Escherichia coli (E. coli) detection. The size and shape of the alkyne-modified FeO@Au NPs were 110 nm and urchin-like, respectively. The azide-functionalized Au QDs were spherical in shape, and their size was ~5 nm. The absorbance and emission peaks of FeO@Au NPs and Au QDs were located at 526 nm and 520 nm, respectively. The nanozymatic activity of both materials significantly improved in the presence of Cu, E. coli, and 3,3',5,5'-tetramethylbenzidine (TMBZ)/HO mixture. Here, the bacterial metabolic product Cu was utilized to trigger the click reaction between alkyne-modified FeO@Au NPs and azide-functionalized Au QDs which enables magnetic separation. The calculated limits of detection (LODs) of E. coli were as high as 5 CFU/mL and 25 CFU/mL in the PBS buffer and spiked raw meat samples, respectively. The proposed nanozymatic E. coli assay was selective in the presence of other bacteria.
用于增强基于纳米酶的大肠杆菌（E. coli）检测的细菌辅助点击化学。炔基修饰的FeO@Au纳米颗粒的尺寸和形状分别为110纳米和海胆状。叠氮化物功能化的金量子点呈球形，其尺寸约为5纳米。FeO@Au纳米颗粒和金量子点的吸收峰和发射峰分别位于526纳米和520纳米处。在铜、大肠杆菌以及3,3',5,5'-四甲基联苯胺（TMBZ）/HO混合物存在的情况下，这两种材料的纳米酶活性均显著提高。在此，利用细菌代谢产物铜引发炔基修饰的FeO@Au纳米颗粒与叠氮化物功能化的金量子点之间的点击反应，从而实现磁分离。在PBS缓冲液和加标的生肉样品中，计算得出的大肠杆菌检测限分别高达5 CFU/mL和25 CFU/mL。所提出的基于纳米酶的大肠杆菌检测方法在存在其他细菌的情况下具有选择性。
