Liu Zhiyi, Cai Yubing, Chen Xu, Cang Yan, Yu Jialiang, Shaaban Muhammad, Cai Yajun, Peng Qi-An
School of Resources and Environment, Wuhan Textile University, Wuhan 430200, China.
College of Agriculture, Henan University of Science and Technology, Luoyang, China.
Curr Res Microb Sci. 2025 Apr 8;8:100388. doi: 10.1016/j.crmicr.2025.100388. eCollection 2025.
Soil provides a habitat for microorganisms that can mitigate metal contamination. This study presents BC4 strain, which shows significant potential for metal pollution remediation. This bacterium achieved a 98.6 % reduction in Chromium (VI) concentrations from 300 mg/L to negligible levels under specific conditions (pH 8, 37 °C, and 120 rpm agitation) in LB medium. The complete genome of BC4 was sequenced using Oxford Nanopore Technology, revealing a circular chromosome and a plasmid with a total of 5537,675 base pairs and a + content of 35.44 %. Fourteen genes critical for Cr metabolism were identified. qRT-PCR demonstrated that under low Cr(VI) stress, two genes, and , were up-regulated, indicating their role in Cr resistance. The genome revealed gene clusters essential for resilience against various metals, including chromium, arsenic, copper, manganese, and cadmium, as well as for synthesizing secondary metabolites crucial for survival and adaptation. Additionally, genes associated with biopolymer synthesis were identified, emphasizing the organism's diverse genetic capabilities. This genomic study led to the submission of the complete genome to GenBank (CP101135), enhancing the understanding and potential of BC4 in chromium remediation and environmental restoration.
土壤为可减轻金属污染的微生物提供了栖息地。本研究介绍了BC4菌株，其在金属污染修复方面显示出巨大潜力。在LB培养基中特定条件下（pH 8、37°C和120 rpm搅拌），该细菌使六价铬浓度从300 mg/L降低了98.6%，降至可忽略不计的水平。使用牛津纳米孔技术对BC4的完整基因组进行了测序，揭示了一个环状染色体和一个质粒，共有5537675个碱基对，GC含量为35.44%。鉴定出了14个对铬代谢至关重要的基因。qRT-PCR表明，在低六价铬胁迫下，两个基因和上调，表明它们在抗铬方面的作用。基因组揭示了对包括铬、砷、铜、锰和镉在内的各种金属具有抗性以及合成对生存和适应至关重要的次级代谢产物所必需的基因簇。此外，还鉴定出了与生物聚合物合成相关的基因，强调了该生物体多样的遗传能力。这项基因组研究导致将完整基因组提交给GenBank（CP101135），增进了对BC4在铬修复和环境恢复方面的理解及潜力。
