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一种处理木薯废料发酵的新型细菌方法：降低氰化物毒性并提高质量以确保牲畜饲料安全。

A novel bacterial approach to cassava waste fermentation: Reducing cyanide toxicity and improving quality to ensure livestock feed safety.

作者信息

Triani Hera Dwi, Yuniza Ahadiyah, Marlida Yetti, Husmaini Husmaini, Astuti Wulansih Dwi, Yanti Gusri

机构信息

Departement of Feed and Nutrition, Faculty of Animal Husbandry, Andalas University, Padang, Indonesia.

Faculty of Science, Social and Education, Prima Nusantara Bukittinggi University, Bukittinggi, Indonesia.

出版信息

Open Vet J. 2025 Mar;15(3):1358-1369. doi: 10.5455/OVJ.2025.v15.i3.27. Epub 2025 Mar 31.

DOI:10.5455/OVJ.2025.v15.i3.27
PMID:40276207
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC12017703/
Abstract

BACKGROUND

Indonesia has a high production of cassava, and cassava waste has significant potential as an alternative feed. However, the high levels of hydrogen cyanide (HCN) and crude fiber in cassava waste pose safety risks for its use as animal feed.

AIM

This study aimed to identify the morphology and molecular characteristics of cyanolytic and cellulolytic bacteria from cassava waste and evaluate their potential in reducing cyanide levels and improving its nutritional value to ensure feed safety.

METHODS

The first step of this research involved the isolation and screening of cyanolytic and cellulolytic bacteria from cassava waste using DNA sequence homology analysis and constructing a phylogenetic tree. The second step evaluated the potential of the identified bacteria to improve cassava waste as a safe feed. The bacteria were used as inoculants in cassava waste fermentation, employing a factorial, completely randomized design with two factors: types of inoculants and fermentation duration. Data were analyzed using analysis of variance followed by Duncan's Multiple Range Test.

RESULTS

This study identified two novel bacterial strains, namely HT3, and HT1. The application of these bacteria as inoculants in cassava waste fermentation at different durations significantly reduced cyanide content, crude fiber, and pH, while significantly increasing protein content. This improved the quality of cassava feed as a safe feed.

CONCLUSION

Cassava waste fermented for 15 days with HT1 produced the best feed quality and safety, with the lowest HCN and crude fiber and high crude protein content.

摘要

背景

印度尼西亚木薯产量很高，木薯废料作为替代饲料具有巨大潜力。然而，木薯废料中高含量的氰化氢（HCN）和粗纤维对其用作动物饲料构成安全风险。

目的

本研究旨在鉴定木薯废料中氰分解菌和纤维素分解菌的形态及分子特征，并评估它们在降低氰化物含量和提高其营养价值以确保饲料安全方面的潜力。

方法

本研究的第一步涉及使用DNA序列同源性分析从木薯废料中分离和筛选氰分解菌和纤维素分解菌，并构建系统发育树。第二步评估已鉴定细菌改善木薯废料成为安全饲料的潜力。将这些细菌用作木薯废料发酵的接种剂，采用析因完全随机设计，有两个因素：接种剂类型和发酵持续时间。数据采用方差分析，随后进行邓肯多重极差检验。

结果

本研究鉴定出两株新细菌菌株，即HT3和HT1。在不同发酵持续时间将这些细菌用作木薯废料发酵的接种剂，显著降低了氰化物含量、粗纤维和pH值，同时显著提高了蛋白质含量。这改善了木薯饲料作为安全饲料的质量。

结论

用HT1发酵15天的木薯废料产生了最佳的饲料质量和安全性，氰化氢和粗纤维含量最低，粗蛋白含量高。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0d5e/12017703/4df7f318dff2/OpenVetJ-15-1358-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0d5e/12017703/eedd8c758f40/OpenVetJ-15-1358-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0d5e/12017703/5a8f6a4eee5d/OpenVetJ-15-1358-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0d5e/12017703/9573ccd18e69/OpenVetJ-15-1358-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0d5e/12017703/6d33d160a657/OpenVetJ-15-1358-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0d5e/12017703/83c2abe7f5e2/OpenVetJ-15-1358-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0d5e/12017703/4df7f318dff2/OpenVetJ-15-1358-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0d5e/12017703/eedd8c758f40/OpenVetJ-15-1358-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0d5e/12017703/5a8f6a4eee5d/OpenVetJ-15-1358-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0d5e/12017703/9573ccd18e69/OpenVetJ-15-1358-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0d5e/12017703/6d33d160a657/OpenVetJ-15-1358-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0d5e/12017703/83c2abe7f5e2/OpenVetJ-15-1358-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0d5e/12017703/4df7f318dff2/OpenVetJ-15-1358-g006.jpg

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