Li Jiaqi, Huang Yuanli, Wang Yalu, Han Qianqian
National Institutes for Food and Drug Control, Beijing 100050, China.
Department of Pharmaceutics, China Pharmaceutical University, Nanjing 211198, China.
Gels. 2025 Mar 22;11(4):226. doi: 10.3390/gels11040226.
Wound healing is a complex biological process involving inflammation, proliferation, and remodeling phases. Effective healing is essential for maintaining skin integrity, driving the need for advanced materials like hydrogels, known for their high water retention and tunable mechanical properties. In this study, we synthesized a biocompatible composite hydrogel composed of γ-polyglutamic acid (γ-PGA) and ε-polylysine (ε-PL) through a Schiff base reaction, forming a stable crosslinked network. Its physicochemical properties, including rheological behavior and swelling capacity, were systematically evaluated. Biocompatibility was assessed via in vitro hemolysis and cytotoxicity assays, and in vivo testing was performed using a full-thickness skin defect model in Sprague Dawley (SD) rats to evaluate wound-healing efficacy. The PGA-PL hydrogel demonstrated excellent physicochemical properties, with a maximum swelling ratio of 65.6%, and biocompatibility as evidenced by low hemolysis rates (<5%) and high cell viability (>80%). It promoted wound healing by inhibiting the inflammatory response, reducing levels of the inflammatory cytokine IL-6, enhancing angiogenesis, and accelerating collagen deposition. The hydrogel showed complete biodegradation within 21 days in vivo without inducing a significant inflammatory response and significantly accelerated wound healing, achieving an 86% healing rate within 7 days compared to 67% in the control group. The PGA-PL composite hydrogel exhibits excellent mechanical strength and biocompatibility, and its effective wound-healing capabilities lay the groundwork for future development and optimization in various tissue engineering applications.
伤口愈合是一个复杂的生物学过程，涉及炎症、增殖和重塑阶段。有效的愈合对于维持皮肤完整性至关重要，这推动了对先进材料（如水凝胶）的需求，水凝胶以其高保水性和可调机械性能而闻名。在本研究中，我们通过席夫碱反应合成了一种由γ-聚谷氨酸（γ-PGA）和ε-聚赖氨酸（ε-PL）组成的生物相容性复合水凝胶，形成了稳定的交联网络。系统评估了其物理化学性质，包括流变行为和溶胀能力。通过体外溶血和细胞毒性试验评估生物相容性，并使用Sprague Dawley（SD）大鼠的全层皮肤缺损模型进行体内测试，以评估伤口愈合效果。PGA-PL水凝胶表现出优异的物理化学性质，最大溶胀率为65.6%，并且具有生物相容性，低溶血率（<5%）和高细胞活力（>80%）证明了这一点。它通过抑制炎症反应、降低炎症细胞因子IL-6水平、促进血管生成和加速胶原蛋白沉积来促进伤口愈合。该水凝胶在体内21天内完全生物降解，未引发明显的炎症反应，并显著加速伤口愈合，与对照组的67%相比，7天内愈合率达到86%。PGA-PL复合水凝胶具有优异的机械强度和生物相容性，其有效的伤口愈合能力为未来各种组织工程应用的开发和优化奠定了基础。
