Seo Hochan, Park Jae Yong, You Hee Sang, Kim Beom Jin, Kim Jae Gyu
Laboratory of Gastrointestinal Mucosal Immunology, Chung-Ang University College of Medicine, Seoul 06974, Republic of Korea.
Department of Internal Medicine, Chung-Ang University College of Medicine, Seoul 06974, Republic of Korea.
J Pers Med. 2025 Apr 4;15(4):144. doi: 10.3390/jpm15040144.
: Gastric cancer treatments can lead to significant alterations to patients' gastrointestinal microbiome. However, differences in microbial impacts between gastrectomy and endoscopic submucosal dissection (ESD) remain underexplored. This study investigates how these treatments influence microbial diversity and composition in patients with stage I gastric cancer. : Patients with pathologically confirmed stage I gastric cancer were recruited from Chung-Ang University Hospital between December 2016 and December 2019. This study analyzed fecal samples from 13 patients (ESD: = 5; gastrectomy: = 8) before and after treatment using 16S rRNA gene sequencing. Microbial diversity indices and taxonomic composition were compared, with follow-up extending up to two years. : In the total cohort, alpha diversity significantly decreased post-treatment ( < 0.05), and beta diversity analysis showed distinct clustering between pre- and post-treatment samples ( < 0.05). At the genus level, Bacteroides significantly decreased ( < 0.05), while Lactobacillus, Bifidobacterium, and Blautia showed significant increases ( < 0.05). Comparative analyses revealed that in the ESD group, alpha diversity remained unchanged, although beta diversity showed significant clustering ( < 0.05), without notable changes in major taxa. In contrast, surgical resection resulted in a significant reduction in alpha diversity ( < 0.05) and beta diversity clustering ( < 0.05), with increased abundances of Streptococcus and Blautia and decreased abundance of Bacteroides ( < 0.05). : Surgical gastrectomy exerts significant effects on microbiome diversity and composition, while ESD has a more limited impact. These findings underscore the importance of considering microbiome changes in postoperative management.
胃癌治疗可导致患者胃肠道微生物群发生显著改变。然而，胃切除术和内镜黏膜下剥离术（ESD）对微生物的影响差异仍未得到充分研究。本研究调查了这些治疗方法如何影响I期胃癌患者的微生物多样性和组成。：2016年12月至2019年12月期间，从中央大学医院招募了病理确诊为I期胃癌的患者。本研究使用16S rRNA基因测序分析了13例患者（ESD组：n = 5；胃切除术组：n = 8）治疗前后的粪便样本。比较了微生物多样性指数和分类组成，随访时间长达两年。：在整个队列中，治疗后α多样性显著降低（P < 0.05），β多样性分析显示治疗前后样本之间存在明显聚类（P < 0.05）。在属水平上，拟杆菌属显著减少（P < 0.05），而乳酸杆菌属、双歧杆菌属和布劳特氏菌属显著增加（P < 0.05）。比较分析显示，在ESD组中，α多样性保持不变，尽管β多样性显示出显著聚类（P < 0.05），主要分类群无明显变化。相比之下，手术切除导致α多样性显著降低（P < 0.05）和β多样性聚类（P < 0.05），链球菌属和布劳特氏菌属丰度增加，拟杆菌属丰度降低（P < 0.05）。：手术胃切除术对微生物群多样性和组成有显著影响，而ESD的影响较为有限。这些发现强调了在术后管理中考虑微生物群变化的重要性。
