Laguerre Hélène, Noël Cyril, Jégou Camille, Fleury Yannick, Le Chevalier Patrick
Laboratoire de Biotechnologie et Chimie Marines, LBCM, EMR-CNRS 6076, University of Brest, F-29000 Quimper, France.
IFREMER-IRSI-Service de Bioinformatique (SeBiMER), 1625 Route de Sainte-Anne, F-29280 Plouzané, France.
Biology (Basel). 2025 Apr 16;14(4):430. doi: 10.3390/biology14040430.
In this study, the bacterial communities of the cœlomic microbiota were characterized in three Echinoderms: the deposit feeder sea Cucumber , the herbivorous sea Urchin , and the carnivorous sea Star . Samples were collected from the same habitat in the Glénan Archipelago (Brittany, France) at different times for 2 years. The cœlomic microbiota were analyzed by targeted metagenomic with V4-16S metabarcoding and by a culturable approach with the isolation of strains and antimicrobial activity assays. Most of the OTUs of the cœlomic microbiota were affiliated with the phylum Proteobacteria and, notably, five orders: Burkholderiales, Flavobacteriales, Alteromonadales, Vibrionales and Pseudomonadales. Significant differences were observed regarding richness, biodiversity and composition between species and sampling dates. They could be explained by sub-abundant taxa that represented the global diversity. Cœlomic microbiota also revealed shared and unshared bacterial communities, validating a potential "specific" microbiota among the three Echinoderm species. Moreover, significant variations of the microbiota occurred among the sampling dates, suggesting a plasticity and, thus, a potential selection of these microbiota. Finally, out of the 831 bacterial strains isolated from culturable microbiota, 20 strains exhibited antibacterial activities, most of them assigned to the genera and .
在本研究中，对三种棘皮动物的体腔微生物群的细菌群落进行了表征：沉积食性的海参、草食性的海胆和肉食性的海星。在两年的不同时间从法国布列塔尼格莱南群岛的同一栖息地采集样本。通过靶向宏基因组学结合V4-16S元条形码分析以及通过分离菌株和抗菌活性测定的可培养方法对体腔微生物群进行分析。体腔微生物群的大多数操作分类单元（OTU）隶属于变形菌门，特别是五个目：伯克霍尔德菌目、黄杆菌目、交替单胞菌目、弧菌目和假单胞菌目。在物种和采样日期之间，在丰富度、生物多样性和组成方面观察到显著差异。这些差异可以由代表全球多样性的亚丰富分类群来解释。体腔微生物群还揭示了共享和非共享的细菌群落，证实了这三种棘皮动物物种之间存在潜在的“特定”微生物群。此外，微生物群在采样日期之间出现了显著变化，表明这些微生物群具有可塑性，因此具有潜在的选择性。最后，从可培养微生物群中分离出的831株细菌菌株中，有20株表现出抗菌活性，其中大多数属于 属和 属。
