从海参中分离出的新种。

sp. nov. isolated from the sea cucumber .

作者信息

Yvin Marion, Mühle Estelle, Chesneau Olivier, Laguerre Hélène, Brillet Benjamin, Fleury Yannick, Jégou Camille, Kämpfer Peter, Lipski André, Criscuolo Alexis, Clermont Dominique, Le Chevalier Patrick

机构信息

Université de Brest, Laboratoire de Biotechnologie et Chimie Marines, LBCM, EMR-CNRS 6076, F-29000 Quimper, France.

Université Bretagne Sud, IRDL, UMR CNRS 6027, F-56100 Lorient, France.

出版信息

Int J Syst Evol Microbiol. 2025 Feb;75(2). doi: 10.1099/ijsem.0.006601.

DOI:10.1099/ijsem.0.006601

PMID:39999156

Abstract

Two motile bacterial strains, designated as cfHf56-1 and SW 252, were isolated from the coelomic fluid of and from the surrounding seawater at the animal sampling site, respectively. The sea cucumber was collected in the Glénan archipelago (Brittany, France). Strains cfHf56-1 and SW 252 were Gram-stain-negative, non-spore-forming and rod-shaped bacteria. Colonies made on marine agar plates were brown in color. The pH and temperature ranges for growth were 7-8 and 18-30 °C, respectively, in marine broth. The major fatty acids were 16 : 1 cis9 and 16 : 0. Phylogenetic analyses evidenced that both strains belong to a novel species in the genus . The strains were closely related to the type strains of , and with 75-78 % ANI and 19-21 % dDDH values. In this context, cfHf56-1 (= CIP 111854 = CECT 30642) is considered as the type strain of the novel species for which the name sp. nov. is proposed. The genome of the type strain is characterized by a size of 5.1 Mbp and a G+C content of 40.5%.

摘要

从海参体腔液和动物采样点周围海水中分别分离出两株运动性细菌菌株，分别命名为cfHf56-1和SW 252。海参采自法国布列塔尼的格莱南群岛。菌株cfHf56-1和SW 252为革兰氏阴性、无芽孢、杆状细菌。在海洋琼脂平板上形成的菌落呈褐色。在海洋肉汤中生长的pH值和温度范围分别为7-8和18-30°C。主要脂肪酸为16:1 cis9和16:0。系统发育分析表明，这两个菌株属于该属中的一个新物种。这些菌株与的模式菌株密切相关，ANI值为75-78%，dDDH值为19-21%。在此背景下，cfHf56-1（=CIP 111854 = CECT 30642）被视为该新物种的模式菌株，为此提议命名为 sp. nov.。模式菌株的基因组大小为5.1 Mbp，G+C含量为40.5%。