Namisy Ahmed, Chen Shu-Yun, Sritongkam Benjapon, Unartngam Jintana, Thanarut Chinnapan, Chung Wen-Hsin
Department of Plant Pathology, National Chung Hsing University, Taichung 402, Taiwan.
Department of Agronomy, National Chung Hsing University, Taichung 402, Taiwan.
Plants (Basel). 2025 Apr 9;14(8):1168. doi: 10.3390/plants14081168.
Fusarium wilt in bitter gourd caused by f. sp. (Fomo) is a severe plant disease that affects the world's bitter gourd ( L.) cultivation. This study evaluated nine luffa hybrids for their performance as rootstocks with bitter gourd to control f. sp. (Folu) isolate Fomh16 and Fomo isolate Fomo33. In the first evaluation, five hybrids (LF1, LF2, LF3, LF15, and LF16) exhibited resistance to the Fomh16 isolate and showed no symptoms. One hybrid, LF10, was resistant with a mean disease rating (MDR) of 0.9 at 28 days post-inoculation (dpi). Seven luff hybrids that displayed resistant and moderate resistance in the first evaluation were used as rootstocks with susceptible bitter gourd cultivars. Five rootstocks exhibited high resistance to Fomh16 and Fomo33 isolates, with their MDR ranging from 0.0 to 0.7. In addition, the findings revealed that both isolates could colonize the vascular bundle of all resistant luffa rootstocks at 28 dpi. However, the Fomo33 isolate could extend and colonize the vascular bundle of bitter gourd scion when grafted only with rootstock LF5 and LF11. The quantitative PCR results indicated that there were significant differences in the amount of the Fomo33 DNA between the bitter gourd grafted onto LF15 and LF16 rootstocks and the self-grafted plants; however, the pathogen cannot be detected in the bitter gourd scions grafted with resistant rootstocks. These findings provide valuable resistant sources that can be used as rootstocks to manage Fusarium wilt disease in bitter gourd.
由尖孢镰刀菌苦瓜专化型（Fomo）引起的苦瓜枯萎病是一种严重的植物病害，影响着全球苦瓜（Momordica charantia L.）的种植。本研究评估了9个丝瓜杂交种作为苦瓜砧木对尖孢镰刀菌丝瓜专化型（Folu）分离株Fomh16和Fomo分离株Fomo33的防控效果。在首次评估中，5个杂交种（LF1、LF2、LF3、LF15和LF16）对Fomh16分离株表现出抗性且无症状。一个杂交种LF10具有抗性，接种后28天的平均病情评级（MDR）为0.9。在首次评估中表现出抗性和中度抗性的7个丝瓜杂交种被用作感病苦瓜品种的砧木。5个砧木对Fomh16和Fomo33分离株表现出高抗性，其MDR范围为0.0至0.7。此外，研究结果表明，在接种后28天，两种分离株均可在所有抗性丝瓜砧木的维管束中定殖。然而，只有当与砧木LF5和LF11嫁接时，Fomo33分离株才能扩展并定殖于苦瓜接穗的维管束中。定量PCR结果表明，嫁接到LF15和LF16砧木上的苦瓜与自嫁接植株之间，Fomo33 DNA的量存在显著差异；然而，在嫁接了抗性砧木的苦瓜接穗中未检测到病原菌。这些研究结果提供了宝贵的抗性资源，可作为砧木用于防治苦瓜枯萎病。
