Luo Xi, Wang Hongfei, Wei Yidong, Wu Fangxi, Zhu Yongsheng, Xie Hongguang, Xie Huaan, Zhang Jianfu
Rice Research Institute, Fujian Academy of Agricultural Sciences, Fuzhou 350019, China.
State Key Laboratory of Ecological Pest Control for Fujian and Taiwan' Crops/Key Laboratory of Germplasm Innovation and Molecular Breeding of Hybrid Rice in South China/Fujian Engineering Laboratory of Crop Molecular Breeding/Fujian Key Laboratory of Rice Molecular Breeding/Fuzhou Branch, National Center of Rice Improvement of China/National Engineering Laboratory of Rice/South Base of National Key Laboratory of Hybrid Rice for China, Fuzhou 350003, China.
Plants (Basel). 2025 Apr 14;14(8):1208. doi: 10.3390/plants14081208.
ALOG ( LSH1 and G1) proteins constitute a plant-specific family of transcription factors that play crucial roles in lateral organ development across land plants. Initially identified through forward genetic studies of Arabidopsis LSH1 and rice G1 proteins, ALOG family members have since been functionally characterized in various plant species. However, research focusing on the characteristics and expression patterns of all family members in rice remains relatively limited. In this study, we systematically characterized family genes in rice. Compared to other genes in rice and , the ALOG family genes have a relatively simple structure. The alignment of OsALOG amino acid sequences and analysis of disorder predictions reveal that all members possess conserved ALOG domains, while the conservation of intrinsically disordered regions (IDRs) is relatively low. Four amino acids-alanine, glycine, proline, and serine-are significantly enriched in the IDRs of each ALOG protein. Synteny analysis indicates that most genes have undergone considerable divergence compared to their counterparts in . Bioinformatic analysis of -regulatory elements predicts that family genes contain elements responsive to ABA, light, and methyl jasmonate, although the abundance and composition of these elements vary among different members. The expression patterns associated with the rice floral development of genes can be broadly categorized into two types; however, even within the same type, differences in expression levels, as well as the initiation time and duration of expression, were observed. These results provide a comprehensive understanding of the structural characteristics and expression patterns of members in rice.
ALOG（LSH1和G1）蛋白构成了一个植物特有的转录因子家族，在陆生植物的侧生器官发育中发挥着关键作用。ALOG家族成员最初是通过对拟南芥LSH1和水稻G1蛋白的正向遗传学研究鉴定出来的，此后已在多种植物物种中进行了功能表征。然而，针对水稻中所有家族成员的特征和表达模式的研究仍然相对有限。在本研究中，我们系统地表征了水稻中的家族基因。与水稻和中的其他基因相比，ALOG家族基因的结构相对简单。OsALOG氨基酸序列的比对和无序预测分析表明，所有成员都具有保守的ALOG结构域，而内在无序区域（IDR）的保守性相对较低。四种氨基酸——丙氨酸、甘氨酸、脯氨酸和丝氨酸——在每个ALOG蛋白的IDR中显著富集。共线性分析表明，与中的对应基因相比，大多数基因已经发生了相当大的分化。对调控元件的生物信息学分析预测，家族基因包含对脱落酸（ABA）、光和茉莉酸甲酯有响应性的元件，尽管这些元件的丰度和组成在不同成员之间有所不同。与水稻花发育相关的基因的表达模式大致可分为两种类型；然而，即使在同一类型中，也观察到了表达水平、起始时间和表达持续时间的差异。这些结果为全面了解水稻中成员的结构特征和表达模式提供了依据。
