Soyama K, Ono E
Clin Chim Acta. 1985 Jul 15;149(2-3):149-54. doi: 10.1016/0009-8981(85)90328-6.
We describe a new, simple, fluorometric assay for D-arabinitol in serum. The method is based on oxidation of D-arabinitol by D-arabinitol dehydrogenase (EC 1.1.1.11), with the concomitant reduction of NAD. The initial rate of NAD reduction, which is proportional to the D-arabinitol content of serum, can be measured with a recording spectrofluorometer. Sensitivity, specificity, recovery and reproducibility experiments gave satisfactory results. The proposed method is suitable for clinical use, and may be helpful in the diagnosis of invasive candidiasis.
我们描述了一种用于检测血清中D -阿拉伯糖醇的新型、简单的荧光测定法。该方法基于D -阿拉伯糖醇脱氢酶（EC 1.1.1.11）对D -阿拉伯糖醇的氧化作用，同时伴有NAD的还原。NAD还原的初始速率与血清中D -阿拉伯糖醇的含量成正比，可通过记录式荧光分光光度计进行测量。灵敏度、特异性、回收率和重现性实验均取得了满意的结果。所提出的方法适用于临床应用，可能有助于侵袭性念珠菌病的诊断。
