Huang Tianhua, Cao Shiran, Li Xiaohan, Wang Chuhan, Peng Xiawei
School of Biological Sciences and Technology, Beijing Forestry University, Beijing 100083, China.
Institute of Tree Development and Genome Editing, Beijing Forestry University, Beijing 100083, China.
Microorganisms. 2025 Apr 11;13(4):880. doi: 10.3390/microorganisms13040880.
Antimony-resistant bacteria are potential natural resources for the bioremediation of mining soil pollution. A sp. 64 strain was isolated from antimony-contaminated soil. To enhance its Sb resistance abilities, this strain was transported into space aboard the Shenzhou spacecraft for space breeding, resulting in a mutant strain, sp. D9. The whole genomes of sp. 64 and mutant strain sp. D9 were sequenced, revealing the genomic information for the bacterium. Comparative genomic analysis was then carried out to identify differential functional genes. The adsorption conditions for Sb(III) were optimized and refined. Further, Fourier transform infrared spectroscopy (FTIR) was used to determine the adsorption of antimony. Results show that strain D9 exhibits a higher tolerance to Sb(III), and Sb resistance genes were identified in both sp. 64 and D9. Analysis of the differential functional genes indicated that the increased copy number of X may lead to a higher lipid content in the cell membrane, thereby enhancing the cell's resistance to heavy metals. Mutant strain D9 exhibited better biosorption capacity compared to strain 64. FTIR studies showed that key functional groups, including -OH, C-N, C-H, and C-O, are likely to have participated in Sb(III) biosorption. Further study of the differential functional genes could provide a basis for future research and the subsequent development of technologies for the remediation of Sb-contaminated sites.
抗锑细菌是修复采矿土壤污染的潜在自然资源。从锑污染土壤中分离出一株A.sp.64菌株。为提高其抗锑能力，该菌株搭载神舟飞船进入太空进行太空育种，得到突变菌株A.sp.D9。对A.sp.64和突变菌株A.sp.D9进行全基因组测序，揭示了该细菌的基因组信息。随后进行比较基因组分析以鉴定差异功能基因。对Sb(III)的吸附条件进行了优化和细化。此外，利用傅里叶变换红外光谱（FTIR）测定锑的吸附情况。结果表明，菌株D9对Sb(III)具有更高的耐受性，且在A.sp.64和D9中均鉴定出抗锑基因。对差异功能基因的分析表明，X拷贝数的增加可能导致细胞膜中脂质含量升高，从而增强细胞对重金属的抗性。与菌株64相比，突变菌株D9表现出更好的生物吸附能力。FTIR研究表明，包括-OH、C-N、C-H和C-O在内的关键官能团可能参与了Sb(III)的生物吸附。对差异功能基因的进一步研究可为未来研究以及后续开发锑污染场地修复技术提供依据。
