Moreira Victor Hugo, Berbert Lidiane Coelho, Adesoji Ayodele Timilehin, Bianco Kayo, Cavalcante Janaina Japiassu Vasconcelos, Pellegrino Flávia Lúcia Piffano Costa, Albano Rodolpho Mattos, Clementino Maysa Mandetta, Cardoso Alexander Machado
Department of Biology, Rio de Janeiro State University, Rio de Janeiro 20550-013, Brazil.
Department of Microbiology, Federal University, Dutsin-Ma 821101, Nigeria.
Microorganisms. 2025 Apr 13;13(4):897. doi: 10.3390/microorganisms13040897.
The increasing prevalence and dissemination of multidrug-resistant bacteria represent a serious concern for public health. is a pathogenic microorganism that causes a wide spectrum of diseases in fish and humans and is often associated with aquatic environments and isolated from foods and animals. Here, we present the isolation and characterization of the V15 strain isolated from a drinking water storage tank in Rio de Janeiro, Brazil. The V15 strain has a genome length of 4,443,347 bp with an average G + C content of 61.78% and a total of 4028 open reading frames. Its genome harbors eight types of antibiotic resistance genes (ARGs) involving resistance to beta-lactamases, macrolides, and quinolones. The presence of , , and mutations in were detected. In addition, other ARGs (, , , and ) and multidrug efflux pumps (such as ), along with several resistance determinants and 106 genes encoding virulence factors, including adherence (polar and lateral flagella), secretion (T2SS, T6SS), toxin (), and stress adaptation () systems, were observed. The genome sequence reported here provides insights into antibiotic resistance, biofilm formation, evolution, and virulence in strains, highlighting the need for more public health attention and the further monitoring of drinking water systems. Also, the results of physiological and phylogenetic data, average nucleotide identity (ANI) calculation, and digital DNA-DNA hybridization (dDDH) analysis support the inclusion of the strain V15 in the genus as a new subspecies with the proposed name subsp. subsp. nov. (V15 = P53320). This study highlights the genomic plasticity and pathogenic potential of within household drinking water systems, calling for the revision of water treatment protocols to address biofilm-mediated resistance and the implementation of routine genomic surveillance to mitigate public health risks.
多重耐药菌的日益流行和传播是公共卫生领域的一个严重问题。[具体细菌名称未给出]是一种致病微生物，可在鱼类和人类中引发多种疾病，常与水生环境相关，且可从食品和动物中分离出来。在此，我们展示了从巴西里约热内卢一个饮用水储存罐中分离出的V15菌株的分离及特性描述。V15菌株的基因组长度为4,443,347 bp，平均G + C含量为61.78%，共有4028个开放阅读框。其基因组含有8种抗生素抗性基因（ARGs），涉及对β-内酰胺酶、大环内酯类和喹诺酮类的抗性。检测到[相关基因名称未给出]中存在[相关突变名称未给出]、[相关突变名称未给出]和[相关突变名称未给出]突变。此外，还观察到其他ARGs（[相关基因名称未给出]、[相关基因名称未给出]、[相关基因名称未给出]和[相关基因名称未给出]）和多药外排泵（如[相关泵名称未给出]），以及几个抗性决定因素和106个编码毒力因子的基因，包括黏附（极性和侧向鞭毛）、分泌（T2SS、T6SS）、毒素（[相关毒素名称未给出]）和应激适应（[相关适应名称未给出]）系统。此处报道的基因组序列为[具体细菌名称未给出]菌株的抗生素抗性、生物膜形成、进化和毒力提供了见解，凸显了需要更多公共卫生关注以及对饮用水系统进行进一步监测。此外，生理和系统发育数据、平均核苷酸同一性（ANI）计算以及数字DNA-DNA杂交（dDDH）分析的结果支持将V15菌株归入[具体细菌属名未给出]属，作为一个新亚种，提议命名为[具体细菌属名未给出] subsp. [具体细菌种名未给出] subsp. nov.（V15 = P53320）。本研究突出了[具体细菌名称未给出]在家庭饮用水系统中的基因组可塑性和致病潜力，呼吁修订水处理方案以应对生物膜介导的抗性，并实施常规基因组监测以降低公共卫生风险。
