Roy Anamika, Roy Pantu Kumar, Cho Sung Rae, Park Shin Young
Department of Seafood Science and Technology, Institute of Marine Industry, Gyeongsang National University, Tongyeong 53064, Republic of Korea.
South Sea Fisheries Research Institute, National Institute of Fisheries Science, Yeosu 59780, Republic of Korea.
Microorganisms. 2025 Apr 16;13(4):914. doi: 10.3390/microorganisms13040914.
Foodborne illnesses, particularly those caused by subsp. Serovar , present a significant challenge to public health, especially within the seafood industry due to biofilm formation on foods. This study investigated the antibiofilm potential of fucoidan, a sulfated polysaccharide, against subsp. Serovar biofilm on crab and shrimp surfaces. Fucoidan's minimum inhibitory concentration (MIC) was determined to be 150 µg/mL. Sub-MIC (1/8, 1/4, 1/2, and MIC) were evaluated for their impact on inhibition of biofilm formation. Fucoidan treatment resulted in significant, dose-dependent inhibition in biofilm formation, achieving 2.61 log CFU/cm and 2.45 log CFU/cm reductions on crab and shrimp surfaces, respectively. FE-SEM analysis confirmed biofilm disruption and cell membrane damage. Real-time PCR showed the downregulation of quorum-sensing () and virulence (, , and ) genes. These results propose that fucoidan has the ability as a natural antibacterial agent for controlling subsp. Serovar biofilms in seafood processing, thereby enhancing food safety and minimizing contamination.
食源性疾病，尤其是由亚种血清型引起的疾病，对公众健康构成重大挑战，特别是在海产品行业，因为食品上会形成生物膜。本研究调查了岩藻依聚糖（一种硫酸化多糖）对螃蟹和虾表面亚种血清型生物膜的抗生物膜潜力。确定岩藻依聚糖的最低抑菌浓度（MIC）为150μg/mL。评估亚抑菌浓度（1/8、1/4、1/2和MIC）对生物膜形成抑制的影响。岩藻依聚糖处理导致生物膜形成受到显著的剂量依赖性抑制，在螃蟹和虾表面分别实现了2.61 log CFU/cm和2.45 log CFU/cm的减少。场发射扫描电子显微镜（FE-SEM）分析证实了生物膜的破坏和细胞膜损伤。实时聚合酶链反应（PCR）显示群体感应（）和毒力（、和）基因的下调。这些结果表明，岩藻依聚糖有能力作为一种天然抗菌剂，用于控制海产品加工中亚种血清型生物膜，从而提高食品安全并最大限度地减少污染。
